VIII. B, 6 Barber: Dysentertj Bacilli 541 



by M. Neisser - as occurring in Bacterium coli mutabile, the 

 secondary colonies of which had the property of fermenting 

 lactose. The secondary colonies of B. coli mutabile were further 

 studied by Massini,^ Arnold Burk,* Sauerbeck,' Kowalenko," 

 Baerthlein,' and Klein.'* Burri ^ found similar secondary colo- 

 nies in Bacterium coli in2)erfectum. Burri and Duggeli ^'^ and 

 Hiibener ' ' found them in various strains of Bacterium coli or 

 coli-like organisms. R. Miiller'- reported secondary colonies in 

 many different kinds of bacteria on 18 different varieties of 

 carbohydrate media. 



In a typhoid-like organism isolated from urine, Sobernheim 

 and Seligmann " found secondary colonies fermenting lactose 

 in Conradi-Drigalski agar. Penfold" obtained in Bacillus ty- 

 phosus secondary colonies which fermented dulcite. Jacobsen^' 

 found secondary colonies in Conradi agar plates made of an 

 atypical strain of typhoid (Bacterium typhi mutabile), which 

 had caused a typhoid epidemic in Denmark. The original strain 

 varied from typical typhoid bacilli in that it possessed a very low 

 degree of agglutinability to typhoid serum and a delayed acid 

 formation in mannite broth. The secondary colonies gave cul- 

 tures which grew unrestrained on Conradi agar and possessed 

 the agglutinability and fermentation characteristic of typhoid 

 bacilli. Seiffert '" worked with a strain of Bacterium coli which 

 had been made resistant to malachite green and had lost the 

 power of forming red colonies on Endo agar. Secondary colo- 

 nies were obtained from this modified strain which not only had 

 regained the power of producing red cultures on Endo agar, but 

 also exhibited a character not possessed by the original strain — 

 that of fermenting cane sugar. 



In the dysentery and paradysentery group of bacilli, R. 



'Centralbl. /. Bakt. etc., Ref. (1906), 38, Beiheft, 98. 

 "Arch. f. Hyg. (1907), 61, 250. 

 'Ibid. (1908), 65, 235. 



'Centralbl. f. Bakt. etc., Orig. (1909), 50, 572. 

 '' Zeitschr. f. Hyg. u. Infectionskrankk. (1910), 66, 277. 

 'Centralbl. f. Bakt. etc., Orig. (1912), 66, 21. 

 "Zeitschr. /. Hyg. u. Infectionskrankh. (1912), 73, 87. 

 Centralbl. f. Bakt. etc., II Abt. (1910), 28, 321. 

 '"Centralbl. f. Bakt. etc., Orig. (1909), 49, 145. 

 ''Centralbl. f. Bakt. etc., Ref. (1909), 44, Beiheft, 136. 

 '- Miinch. med. Wochenschr. (1909), 56, pt. 1, 885. 

 '"Centralbl. f. Bakt. etc., Ref. (1911), 50, Beiheft, 134. 

 " Journ. Hyg., Cambridge (1911), 11, 30. 

 "Centralbl. f. Bakt. etc., Orig. (1910), 56, 208. 

 '"Zeitschr. f. Hyg. u. Infectionskrankh. (1912), 71, 561. 



