134 BOTANICAL GAZETTE [FEBRUARY 
In very few cases was there any ambiguity in this respect, although 
several malformed zoosporangia were seen. In one of these a 
heavy wall had formed across the neck, leaving only a small pore 
between the neck and the body of the cyst. Several very narrow- 
necked zoosporangia were also observed, but though these resembled 
the resting cysts in shape, they were apparently otherwise normal. 
MICROCHEMICAL REACTIONS.—The two outer walls of the 
resting spores are cellulose, as reported by LAGERHEIM, who used 
chlor-zinc-iodide as a test reagent. With iodine and sulphuric 
acid also they give the cellulose reaction, but were not in my tests 
as deep a blue as the cotton fibers which were used as a check. 
But the endospore is different in character and was unaffected by 
any of the reagents or stains employed. 
LAGERHEIM suspected that there might be chlorophyll in some 
stage of the life cycle, though he was not able to detect it. “The 
plant has more or less red pigment at all stages, but none of my. 
observations gave any ground for supposing chlorophyll to be 
present. 
The red pigment, as reported by LaGERuHErIM, is haemato- 
chrome or some closely related lipochrome. It is colored green 
with iodine in potassium iodide, blue with sulphuric and nitric 
acids, fading away after treatment with the latter. Tests with red 
individuals of Sphaerella under the same cover-glass with Rhodochy- 
trium gave somewhat contradictory results, but showed some 
differences between the pigments of the two. The haematochrome 
of Sphaerella was not dissolved by carbon disulphide, which is a 
solvent for the allied pigment carotin, even after prolonged treat- 
ment, but the pigment of Rhodochytrium was easily dissolved under 
the same conditions. The haematochrome reacted to a weak 
solution of iodine such as is used for testing starch, but the pigment 
of Rhodochytrium remained unchanged until a strong solution of 
iodine was applied, when the characteristic reaction appeared. 
With sulphuric acid also Sphaerella reacted instantly, but drops of 
the red oil of Rhodochytrium remained unchanged for several minutes 
and slowly turned blue. While still inclosed in the unbroken 
spore, the pigment is very resistant to almost all reagents. This 
was first noticed on fixing with chromacetic acid, which fades out 
