302 BOTANICAL GAZETTE [APRIL 
INTERKINESIS.—The heterotypic division presents no unusual 
features, but interkinesis differs markedly from that of the buck- 
wheat. Although a rather definite nuclear membrane is formed, 
the chromosomes show no signs of vacuolation or anastomosis. 
On the contrary, they become arranged around the periphery of 
the nucleus and present at this stage the most satisfactory oppor- 
tunity for counting and comparing the chromosomes (figs. 57 and 58). 
The haploid number is 16. 
Nucleoli appear at this stage and stain with haematoxylin in 
exactly the same way as the chromosomes themselves; in fact 
they were at first mistaken for larger, more regular chromosomes. 
With safranin, however, the nucleoli are clearly differentiated from 
the chromosomes. There may be either one or two nucleoli at this 
stage, but sister nuclei seem to agree in this respect, that is, if one 
daughter nucleus of the first division shows one nucleolus, its sister 
nucleus also has only one, but both may, on the other hand, have 
two nucleoli (figs. 57 and 58). This peculiarity seemed at first to bear 
out the idea that they were ee, and there seems to be a 
, thelong-styled form having 
two nucleoli (fig. 58) and the Siorteatyled form one larger nucleolus 
(fig. 57). This may possibly be due to the different sizes of the 
nuclei in the two forms. 
THE CHROMOSOMES IN THE REDUCTION DIVISION 
Interkinesis, because the chromosomes remain apparently 
unchanged and are arranged in the periphery of the nucleus, affords 
the best opportunity for comparing the chromosomes of the reduc- 
tion division. No difference in the chromosomes that enter into 
the formation of the daughter nuclei could be discovered. In fact, 
both in interkinesis and in the anaphase of the reduction division 
the chromosomes show very little variation in size. A constant 
difference in size between the chromosomes of the long-styled and 
short-styled forms is evident in the anaphase, but this is much less 
marked than in the buckwheat (compare figs. 55 and 56). 
RELATIVE SIZE OF THE POLLEN MOTHER CELLS 
The pollen grains of the two forms differ fully as much in size 
as do the two forms in the buckwheat, but the difference does not 
