376 BOTANICAL GAZETTE [may 
spore bed of the rust; fig. 2 shows the abnormal number of three 
nuclei in one cell; while fig. 3 shows some deformation accompany- 
ing the hyperplastic condition. 
3. PSEDERA TRICUSPIDATA (Sieb. and Zucc.) Rehder, para- 
sitized by Phyllosticta Labruscae Thum.—The normal leaf tissue 
is similar to that of the Gaylussacia described above, and the patho- 
logic changes are very much alike in the two plants. The brown- 
ing and collapse of the epidermal, palisade, and sponge cells are 
common to both. The early disappearance of nuclei and chloro- 
plasts is also similar in the two. There is in this case a granular 
protoplasm in the cells next to the dead area, while the homo- 
geneous brown mass is not formed. The sponge tissue is more 
completely disorganized than the palisade. This plant, collected 
in early July, was growing upon a wall exposed to the morning sun. 
4. SMILAX GLAUCA Walt., parasitized by a member of the 
Phaeodidymae of the Sphaerioidaceae——The leaf parenchyma is 
not differentiated into palisade and sponge tissues. The diseased 
area is badly shrunken and broken down. The fungous perithecia 
are scattered irregularly over this dead area. Because of the 
thorough killing of the host tissue, no special cytologic changes 
could be noted. At the edges of the diseased area the cells of both 
the upper and lower epidermis are turned brown to black, while 
some of the mesophyll cells are filled with a uniformly brown- 
staining material. The intercellular spaces and the primary 
lamellae are often stained very deeply by the hematoxylin. In 
this transition region no cell organs are visible, and whatever is 
left of the protoplast is turned black or brown. This coloration 
sometimes extends to the cell walls as well. 
5. POTENTILLA CANADENSIS L., parasitized by Puccinia Poten- 
tillae Schw. (?).—There is a double parasitism represented here. 
Darluca filum was found growing abundantly upon the spore beds 
of the rust, and at times apparently directly upon the leaf tissue 
of Potentilla. ‘This latter condition, however, may have been due 
to the rust mycelium in the tissues which had not yet produced a 
noticeable spore bed. 
The normal structure of this Potentilla leaf is somewhat more 
complex than that of the Viola described previously. The palisade 
