1915] ROSE—APPLE BARK 57 
tus were fastened. Oxidation, as indicated by rise of mercury 
(negative pressure really), was not complete even at the end of 
seven days, as was well demonstrated by later experiments. 
TABLE I 
MANOMETER READINGS IN EXPERIMENT NO. I; EXTRACT OF HEALTHY BARK, SAMPLE 17 
MANOMETER READINGS CORRECTED AGAINST AN APPA- 
RATUS CONTAINING ONLY WATER 
Day ELAPSED | TEMPERA- 
OF TIME OF TIME IN TURE AT 
TES READING |HOURS AND| TIME OF Extract unchanged H.O 
MINUTE: READING 
8 9 Ir 12 Io 13 
Ist 3:55 P.M ros 36.0 ° 0.00 0.00 | 0.0 
2d Q:30A.M.) 37°36. | 995.646. 15 |—0. 20 |--0.25"| -—0. 20 {--0.20 | 0.9 
2: 30°P 22:35 | 36.0 |—0o,20 |—0.25 |—0.35 |—0.30 |—0.20 | 0.0 
3d 5:00P.M.| 49:05 | 35.0 |—0.60 |—0.60 |—0.66 |—0.60 |—0.60 | 0.0 
4th...| 2:37P.m.| 70:42 | 33.9 |—o0.80 |—0.75 |—0.93 |—0.82 |—0.90 | 0.0 
5:00 P. 97:05 | 34.0 |—0.80 |—0.78 |—0.92 |—0.95 |—0.98 | 0.0 
5th...| 8:50A.M.| 112:55 | 32.0 |—0.95 |—0.90 |—1.08 |—1.04 |—1.08 | 0.0 
6th.. .|11:25A.M.| 139:30 | 34.0 |—1.25 |—1.25 |—1.25 |—1I.15 |—1.30 | 9.0 
7th...| g:00A.M.| 161:05 | 33.0 |—1.40 |—I.35 |—1.35 |—1-30 |—1.40 | 0.0 
t in incubator at 2:55 P.M.; sient and shaken at 3:55 P.M. to mix thoroughly the extract and 
bpccgatel shaken again next morning 
A similar experiment (no. 2) was set up, using the extract of 
diseased bark from another Ben Davis tree in the same orchard. 
The results are given in table II. 
TABLE II 
MANOMETER READINGS seacusie 7 EXPERIMENT NO. 2; EXTRACT OF DISEASED 
RK, SAMPLE I5 
MANOMETER READINGS CORRECTED sage! A TUBE 
CONTAINING ONLY WAT 
TEMPERA- 
Dayor| Trweor | TMEIN | sure at 
TEsT | rEapING | OURS | tie oF Extract unchanged HO 
shai READING Scie i 
MINU 
8 9 ro Ir 12 13 
§:09P.M.| 48:59 | 37.0 |—1.75 |—1.50 |—1.77 |—1-55 |—1-55"| 9-0 
th 4:45P.M.| 72:35 | 36.0 |—1.75 |—1.65 |—1.7 —1.75 | 0.0 
5th 8:18A Se:08'} 36:2 |—5:99 j—-t-75 | 1.93 [t-95 1 ~ °.0 
* Put in incubator at 3:00 P.M.; all apparatus closed and shaken at 4:10 P.M.; shaken again next 
Morning. , wife ctf 
