202 BOTANICAL GAZETTE [SEPTEMBER 
between ethylene treated and untreated tissue; for, as has been 
stated, a given culture was not divided into the two portions until 
72 hours before the end of the cultural period. Considering the 
unavoidable variations which must enter into different cultures, 
the treated and untreated pair of samples from one culture should 
therefore be more comparable than any other combination; for 
example, the treated sample of one culture and the untreated of 
another. Samples I and II, afterward IV and V, VI and VII, 
etc., are directly comparable. 
TABLE I 
TOTAL ALCOHOL-ETHER SOLUBLE SUBSTANCES IN THE ETHYLENE TREATED AND 
UNTREATED TISSUE 
UNTREATED TREATED 
Percent: P t P t: P t: 
No. cd of alcohol- k asahek. gprs a No. - of sata of alcohol- her pe 
sample ether e: . er i 
f soluble leatatle solids essa sitanie ‘epoleble solids 
bo ats 59.70 40,21 5.93) fu 67.63 32.37 8.54 
Wo ae 59.28 40.72 Beg PAV ese, 68.80 31.20 7.82 
Nike 59.04 40.96 Be Nay, 67.43 32.57 8.32 
Boda | ae 59.40 40.60 See - RE, 43 64.98 35.02 8.60 
AV 60.00 40.00 7.801 MAY cats, yy: 32.40 8.30 
Pe as 59.60 40.40 8.34 | XVIII (a)| 68.64 31.36 8.90 
ye 6 TE 59.70 40.30 8.53 | XVIII (d)| 68.76 31.34 8.94 
XXIII 59.66 40.37 8.48 
Mean 59-55 | 40.45 | 8.27 Mean..| 67.70 | 32.30 | 8.48 
Table I gives the results of the separation of the samples into 
alcohol-ether soluble and insoluble portions. Two facts are note- 
worthy: first, that the soluble substances are more abundant in 
the ethylene treated than in the untreated tissue, a difference 
amounting to about 8 per cent; and, secondly, the water content of 
the two tissues is practically the same, being about 91.5 per cent. 
To what substances this difference is due should become clearer 
from the tables to follow. ; 
Table II shows the amount of reducing sugars in the alcohol- 
ether soluble fraction before and after hydrolysis, and also the redu- 
cing power of the insoluble fraction after hydrolysis. These latter 
data cannot be expressed, even approximately, in percentages, 
