1915] HARVEY—ETHYLENE 203 
on account of the probability of a great variety of hydrolyzable 
polysaccharides present. 
The amount of reducing sugars in the soluble fraction is con- 
siderably greater in the treated tissue, although the results of sample 
II are inconsistent. Again, after hydrolysis, the treated tissue 
still shows more reducing sugar, but the difference is less pro- 
nounced, which means that the higher soluble carbohydrates, such 
as the disaccharides, are really less in this tissue than in the un- 
treated. One seems justified in saying that the ethylene treated 
tissue has about 11 per cent more of the lower, and about 3 per cent 
' TABLE If 
CARBOHYDRATES 
‘ ALCOHOL-ETHER 
ALCOHOL-ETHER SOLUBLE FRACTION INSOLUBLE 
FRACTION 
Noe of nd ake tape of Percentage of I ty = in Hyaceger 
0.0 reducing sugars : ncrease by ns 
sample before hydrol- pe i an hydrolysis eer g aon 
— hydrolysis 
ed ee ecda ee ears eee 153-9 
ghee ea Ente acres) Berar eg re Ree mek ar 162.2 
: VII 10.19 15.92 5.83 143.4 
Untreated tissue PPE Gf) Oe er ee: Pee eer ey it ee ek 131.4) 
FEET (DB) uiccs oie cc ba circ de aat ee iai a ey eee Sees 145.2 
XV 12.30 15.70 3.40 140.1 
I 13.84 15.15 Tar 120.7 
Ethylene treated) | IV 23.18 25.50 2.38 124.6 
tise. is ces VI 23.70 25.30 t 109.7 
XIV 20.39 aI.St 1.48 118.8 
* Hydrolysis 5 hours. 
less of the higher soluble sugars than the untreated. The reducing 
power of the alcohol-ether insoluble fraction after hydrolysis is 
clearly less in the treated tissue. The polysaccharides, which are 
likely to be present and which are capable of yielding reducing 
sugars by this acid hydrolysis, are starch, ligno-celluloses, galactans, 
pectins, etc. Microchemical tests show that very little starch is 
present in either tissue. The reducing sugars, therefore, are largely 
from other polysaccharides. An examination of the drawings of 
fig. 2 will aid in interpreting the differences found. Around the 
four leaf traces, mechanical tissue is considerably more abundantly 
