204 BOTANICAL GAZETTE [SEPTEMBER 
developed in the untreated epicotyl. This anatomical difference 
agrees with the findings of KAUFMANN (18) for lupine seedlings 
treated with ether. From a chemical viewpoint the difference 
seems sufficient to account for the greater power of reduction of the 
untreated tissue after hydrolysis, inasmuch as mechanical tissue 
contains a large proportion of rather easily hydrolyzable carbohy- 
drates. That these polysaccharides are not completely hydrolyzed 
by the 2.5 hours’ hydrolysis is shown by comparing samples 
XIII (a) and XIII (6), in which the hydrolyzing time of the latter 
was doubled. 
TABLE III 
No. of sample semi iter’ | babtecs of caenaas 
5.09 2 
VII 16.18 11.4 
Untiented Gesné: 06 sas o. XIII (a) £5.00) Ala sa ewer 
XIII (8) Re a eran eee 
XV Lio) ieee Cae Pec a 
Il EL Bienes PROSE RE oS 
Ethylene treated tissue. ...... IV it. Y 6.22 
VI 4198 8.3 
XIV £1.66 2 leiweleaeenies 
* Hydrolysis 5 hours. 
Table III gives the results of ‘crude fiber’’ determinations. 
“Crude fiber” obviously is a mixture of a large number of sub- 
stances, such as unhydrolyzed protein, cellulose and other poly- 
saccharides, etc. However, this fiber must be largely cellulose 
and protein. Less crude fiber was found in the treated tissue. 
The approximate percentage of cellulose was estimated by sub- 
tracting from “‘crude fiber’’ the total protein present before hydrol- 
ysis, minus the protein rendered soluble by the acid hydrolysis. 
By this method relative differences, at least, should be shown, and 
the results indicate that the treated tissue had about 3 per cent - 
less cellulose than the control. 
In table IV are shown the total nitrogen and the ammonia and 
amino nitrogen, before and after hydrolysis, in the alcohol-ether 
soluble fraction, and the total nitrogen in the alcohol-ether insoluble 
fraction. 
The total nitrogen figures for the former fraction do not show 
a difference between the treated and untreated tissues. Both the 
