1915] HARVEY—ETHYLENE 205 
formol titration and the VAN SLYKE methods show a higher amino 
nitrogen content in the treated tissue before hydrolysis, but after 
hydrolysis the difference does not appear. (Determinations after 
hydrolysis were made only by the VAN SLYKE method.) Since the 
increase of alpha-NH, nitrogen, by hydrolysis, is somewhat more 
in the untreated tissue, it suggests a less amount of polypeptides 
in the treated tissue, a situation for the soluble nitrogenous sub- 
stances corresponding to that of the soluble carbohydrates of this 
fraction. The ammonia-nitrogen results are very variable and no 
conclusions can be drawn from them. 
TABLE IV 
NITROGEN 
ALCOHOL-ETHER 
ALCOHOL-ETHER SOLUBLE FRACTION INSOLUBLE 
FRACTION 
Amino- A 
NH: nitrogen NH nitrogen 
No. of sample Born Peace peu Hes Bad asl PE bewnott Protein 
* yi ‘Ol- * vi r 
ysis ysis ysis ysis 
I oe epee res Pare ety Bee, 2.19 | 13.68 
Vv “ae: hy Bey ees aa es fee ee) t.9% | Xf 93 
Untreated Vil Po Oat etree eee is Eby eed 1.501 9.3 
ti XIII Pamir s Ce an Fe ae ty oe Ba aged lace kw ete we 8 ee 
acvdereat Ss Fs Fae meg Panes ney) cg MO, Pee med Beene) Pear ry 
et ee ere res 0.32 | 1.96 [oe ce cele ewe s|en cece efenenes 
DB GS Seen Cpe m as SOE) Ce rt oe ea meer BBD liv ceee sbawens © 
POP a aes 0.76 | 2.02 | 1.30 | 2.78 |.......|----.- 
II Cee 8 ene Cee Oy Cokie Cee 1.82 |. 12.37 
Ethylene IV Pee epee beat eee oe 1.57 | 9.8% 
Cl leas ea ss[ouweses 6. : 
= teste [a ale Wn i aca, BA) ie, Sa a Riese 
FV Oa epee a hg RE ER) eS Coo 
OOVILE CA) yeas 0.83 | 2.54 | 0.93 | 3-22 |.---+++[---- 
meV Ch) ei 0.40 | 2.41 | 1.25 | 2.98 |..+---.|------ 
* Obtained by the formal-titration method. 
The total nitrogen of the alcohol-ether insoluble fraction is 
less in the treated tissue. By employing the factor 6.25 to the 
nitrogen figures, it is found that the proteins are about 3 per cent 
less in the treated tissue. Although the results at first seem some- 
what inconsistent, they no longer appear so when treated and 
untreated samples of the same culture are compared. 
