206 BOTANICAL GAZETTE [SEPTEMBER 
Table V gives the percentage of ash of the soluble and insoluble 
fractions. No marked difference appears between the two tissues. 
TABLE V 
ASH 
Percentage of ash of the | Percentage of ash of the 
No. of sample alcohol-ether alcohol-ether insoluble 
extract residue 
1 Se eae TAC orators bie di ak ea ee 1.58 
Greate: ase es es eee a ee uit nas weer 1.93 
VII 3.08 1.35 
Ethylene treated It 2.76 1.54 
TSRUE IV goa5 1. At 
VI Re ye 1.47 
Table VI shows the amount of ether soluble substance and the 
free fatty acid value. The figures in parentheses were not deter- 
mined, but calculated on the assumption that the percentage of 
TABLE VI 
Fats 
7, of 
10 
Nise | NaH to 
Dry wt. of | Percentage neutralize 
No. of | Wet wt. of/Dry wt. of sthar él ethane: NaOH to | f, 
sample | tissue gm. | tissue gm. ||. gm.|extractives| © 
{| Sexe 119.57 | 10.043 | (0.220) 
XXIX 171-041 14.417 | (0.353 
(2.2) 
Dota. Pe 
Ethylene treat-{| XX VI 130.45 {| 11.722 | (07140) } (3.2) 1.92 | (13-71) 
dee XXVIII | 264.55 | 22.222 | 0.271 22 § 
ether-extractives was the same in these samples as in others. One 
thing is rather clear from the table, namely that less fat is present 
in the treated tissue, a fact which agrees with the effects of acetylene 
in oily seeds as studied by GRAFE and RICHTER (9). The free acid 
value is of particular interest on account of the claim of IWANOW 
(13) that the free acid value is predetermined by the degree of 
saturation of the fatty acids involved in the fat in question. How- 
ever, the free acid value of the fats in the two tissues was not found 
