256 BOTANICAL GAZETTE [OCTOBER 
form long roots or shoots as will the completely isolated leaf. The 
analogy between the effect of the non-removal of the opposite leaf 
from the stem and of the removal of the opposite bud seems thus 
pretty complete. 
IV. Continuation of these experiments 
We have thus seen that the growth of buds on the stem is one 
factor which inhibits or delays the growth of the notches in the oppo- 
site leaf. We intend to 
show the influence of this 
factor in some further 
observations. 
In all previous experi- 
ments we had cut out 
from a plant a piece of 
stem with only one node. 
If we cut out a piece of 
a stem containing two or 
three nodes (figs. 10, II, 
12, 13) and preserve one 
pair of leaves, the behavior 
of these leaves will be 
different if they are left in ' 
the apical or in the basal 
node of the piece. Figs. 
10-13 illustrate this differ- 
ence. In all cases one OF 
Fics. 10 AND 11 both leaves are partly sub- 
mersed in water, while the 
rest of the preparation is suspended in moist air. In such cases 
new shoots (SS) were formed in a few days from the two apical 
buds of the stem in fig. 11, where the apical leaves had been 
removed and only the basal leaves left; while in specimens like 
fig. 10, where the apical leaves were left, the buds on the stem 
either formed no new shoots or formed them with some delay. 
As a consequence, we notice that in fig. 11 the submersed leaf 
formed at first no shoots, while the submersed leaf in fig. 10 in 
