352 BOTANICAL GAZETTE [NOVEMBER 
On tannin neither species grew even as much as might have 
been expected from the nutriment stored in the agar block. 
On extract “A” a fair growth was made, using up the food 
material stored in the original agar block and forming masses of 
mycelium about 2 cm. across and producing abundant pycnospores. 
On extract “I-X”’ an abundant growth was secured, entirely 
filling the liquid in the flask with a thick growth of mycelium which 
rose above the liquid and produced abundant pycnospores. , 
The second series was made up in strength of 0.2, 0.4, 0.6, 
o.8, and r per cent tannic and gallic acid. These were sown with 
spores of the same fungi used before. In no case was growth made, 
although check sowing on agar showed the spores to be viable. 
Endothia on tannin 
GROWTH OF ENDOTHIA ON COMMERCIAL TANNIN (MERCK) 
The cultures used for this series were made to contain 0.1 per 
cent, o.2 per cent, 0.4 per cent, and by intervals of 0.2 per cent 
up to 2.4 per cent of tannin. In this series of experiments the agar 
remained firm, except in one or two of the cultures containing the 
highest percentages of tannin, in which there was a slight tendency 
to a semifluid condition. No cultures showed sufficient proteid 
digestion to allow the formation of a liquid. The following strains 
of Endothia were used: E. parasitica (STEVENS no. 1158), a Chinese 
strain from the Bureau of Plant Industry,° and a strain designated 
“PP.” of our own isolation from Prospect Park (Brooklyn) 
material; E. radicalis (METCALF no. A, STEVENS no. 2391, a Euro- 
pean strain secured from ANDERSON, and CrinTon’s E. gyrosa 
no. 7677); and E. radicalis mississippiensis (STEVENS nos. 1196 
and 3443). 
Endothia parasitica (American strain) gave a good growth 
of aerial mycelium, varying in amount in direct ratio to the 
percentage of tannin used. Poor growth on check until the end 
of the second week. Yellow color in mycelium at end of first week 
and discoloration of the agar during the second week. Cultures 
originally containing o.2 gm. tannin (2 per cent) were sent to KERR, 
6 This culture was made from material sent directly from China to the Bureau of 
Plant Industry. 
