1915] BRIEFER ARTICLES 413 
noticeable except that germination was apparent up to and including 
the tubes containing 0.6 per cent ether. The second day the culture 
containing 0.3, 0.4, and o.5cc. ether showed accelerated growth, 
while the one with 0.6 per cent showed good growth. On the tenth day 
after inoculation, the cultures with the higher percentages of ether showed 
signs of more vigorous growth, except the two highest members of the 
series, which never germinated. From this time on the results do not 
agree in all details with the earlier stages of the experiment, owing to the 
unavoidable escape of varying amounts of ether from the different 
tubes and the consequent change of percentages. Therefore, it was not 
practicable to keep these cultures containing ether under observation 
for more than ten days or two weeks, a time too short for pycnidial © 
formation in liquid media. 
These results are represented in the accompanying table, in which 
the check is rated 5, and the acceleration of retardation computed on 
this basis. 
TABLE I 
Growts or Endothia ate IN LIQUID MEDIA TO WHICH THE INDICATED AMOUNTS 
R WERE ADDED (AMOUNT IN CC.) 
bs Spec and 0.0 | 0.05 | o.t | 0.2 | 0.3 | 0.4 | 0.5 | 0.6 | 0.7 | 0.8 | 0.9 | 1.0 
Loe ere 5 7 9 2 I ° ° ° ° ° ° ° 
sige 5 7 9 4 | 2 I 2 ° ° ° ° 
eee 5 5 6 5 4 4 3 3 fe) ° ° ° 
Lea Pee 5 5 6 ates a 5 4 3 ° ° ° ° 
ye RD 5 6 7 6 5 5 4 3 I ° ° ° 
a 5 6 6 6 5 5 4 Ey 2 I ° ° 
se 5 7 6 6 5 5 4 3 2 3 ° ° 
It appears that small quantities of ether have a stimulating effect 
on the fungus, quantities of from o. 2 per cent up retard germination, and 
quantities from o.4 per cent up have injurious effect on the growth of 
the fungus. While it is possible to grow the mycelium under the influ- 
ence of ether, the volatile nature of this chemical makes it impossible 
to keep such cultures intact long enough for pycnospores to appear. 
After two to two and one-half weeks the mycelium showed signs of dying 
and pulling away from the glass in the cultures containing the higher 
percentages of ether—MeEtvittE T. Cook and Guy W. WItson, 
Rutgers College and New Jersey Agricultural Experiment Station, New 
Brunswick, N.J. 
