I915] HUTCHINSON—ABIES BALSAMEA 463 
the second set of spindle fibers begins to be differentiated, the 4x 
number of chromosomes are indiscriminately intermingled (figs. 
46-48). Half the number pass to each pole to form the daughter 
nuclei. 
The chromosome count.—Repeated chromosome counts in the 
sporophyte and gametophyte confirm one another in fixing the « 
and 2x numbers for Abies balsamea as 16 and 32. The individual 
chromosomes which appear during the prophase of the division in 
the central cell are shown in figs. 8 and 9. The countis 16. The 
division of the body cell in anaphase gives 39 segments. A recon- 
struction of parts separated by the microtome knife results in a 
count of 32, or 16 passing to each pole. At the time of approxima- 
tion of the chromosomes in the egg, there are 16 pairs (figs. 29-33). 
When segmentation takes place, 32 pairs of segments are present 
(figs. 34-40). In the nucleus represented in figs. 43-45, there are 
72 chromosome pieces; figs. 46-48 show 63 almost complete 
chromosomes, besides a number of ends. Undoubtedly we have 
in each case the 4% number, or 64 chromosomes. 
The daughter nuclei.—During telophase the chromatin strands 
remain remarkably distinct (figs. 49-52). They elongate greatly, 
and become irregularly looped (figs. 50, 52). It would seem that 
each is in contact with the periphery somewhere throughout its 
length. Contraction is followed by an increase in the size of the 
nuclear space; the latter is accompanied by a vacuolization of the 
chromatin (fig. 55). The nuclear outline is still lobed, the lobes 
corresponding to the loops of chromatin. The nuclear membrane 
forms late. As the nucleus continues to enlarge, the chromatin 
becomes still more discontinuous, but the outline of the strands 
may still be readily traced. 
It is to be noted that of all the material which was inclosed by 
the membrane of the egg nucleus, only the chromatin is included 
in the newly organized daughter nuclei. The large vacuolate 
darkly staining bodies, the filaments pervaded by granules, and 
the fibers are all excluded when the membranes inclose the daughter 
nuclei. We may conclude that these materials, although they may 
be found within the nuclear membrane, are not essentially nuclear 
and are not directly concerned in mitosis. They are, at most, 
