Amoeboid cells iu intestinal absorption. 7 



wMch was published later on in the same year. The description is 



as follows (p. 194): 



'■'■Fat absorption. — For the purpose of studying the 

 coiirse which fatty particles take in passing from the cavity 

 of the iütestine into the central lacteals of the villi, an 

 animal is killed three or four hours after a meal composed 

 almost exclusively of fat (it should previously have been allowed 

 to fast for several hours). On opening the abdomen the 

 lacteals in the mesentery will be found filled with chyle, and 

 the cavity of the small intestines occupied by emulsified fat 

 which is undergoing absorption. The intestine is opened at 

 once, and two or three very small pieces of the mucous mem- 

 brane are snipped off and placed in 1 per cent osmic acid 

 Solution. Another minute piece is placed in a drop of serum 

 or aqueous humour and is quickly teased out v^th needles; 

 a piece of hair is added, and the preparation is covered and 

 examined. One of the portions in osmic acid is allowed to 

 remain forty-eight hours in the Solution and is then broken 

 up in water. The others are transferred to dilute Kleinen- 

 berg's Solution (haematoxylin), and when stained throughout 

 are embedded by the cacao-butter process (p. 176). The 

 sections are placed, after the cacao-butter has been extracted 

 from them by warm oil of cloves, flrst in spirit, and then in 

 water and are finally mounted in glycerine. 



In the two teased preparations — serum and osmic — 

 many of the columnar ceUs will be found to contain fatty 

 globules of various sizes (stained black in the osmic pre- 

 paration). Similar but for the most part smaller particles 

 will also be found in the numerous lymphoid corpuscles which 

 are set free from the retiform tissue of the mucous membrane 

 by the process of teasing. In the sections the epithehum cells 

 and the lymph- corpuscles will be observed, in situ, in the 

 same condition, viz : containing blackened fatty particles, and 

 moreover the cleft-like central lacteal in the middle of each 

 viUus will be found to contain similar globules. Hence we 

 infer that the fatty matters are first taken up from the cavity 



