﻿BOTANICAL GAZETTE 



2. As a result of this separation it is possible to eliminate fungi 

 from experiments involving the effect of protozoa upon bacterial 

 activity, by making a subculture from the fungi-free solution of 

 bacteria and protozoa (in the cavity of the agar plate) . 



3. The second method described, that of removing the fungi 

 from the plates as they appeared, is undesirable for our special 

 investigation, for the reason that the bacteria are allowed to multi- 

 ply easily, while the protozoa have no such favorable conditions; 

 consequently, on transferring such a culture to the soil, the protozoa 

 would be at a considerable disadvantage, and their activity would 

 be seriously inhibited if not entirely suppressed. The suggestion 

 may be offered, however, that this method might be employed for 

 obtaining cultures of single types of protozoa, as for example small 

 flagellates or small ciliates. 



This paper represents one phase of the preliminary work 

 undertaken in connection with an investigation of the effect of 

 soil protozoa upon the activity of soil bacteria. Further results 

 on experimentation and a bibliography on soil protozoa and soil 

 sterilization are awaiting publication. 



Rutgers College 

 New Brunswick, N.J. 



