﻿3?o 



BOTANICAL GAZETTE 



The network as it passes to the poles is seen to be not a uniform 

 disk, but to become concave (fig. 55), the concavity pointing to the 

 poles, indicating the direction of the attractive force. In some 

 cases the concavity appears as a cone of granular material on a 

 base of deeply stained tetrahedral masses (fig. 58). Arrived at the 

 poles the network now converges to a spherical shape (figs. 59, 60), 

 the chromatic material tending to become peripheral in position 

 (fig. 62). Irregular masses of it later may be discharged. Fig. 67 

 illustrates the intimate connection of chromatic substance with 

 pyrenoids. Figs. 62-68 show that as many variations mark the 

 beginning of telophase as were seen in prophase. The chromatic 

 masses become reduced in size, while the nucleolus as a reserve 

 body appears in their midst. 



Since the increase in size of the nucleolus is correlative with 

 the reduction in chromatic masses, it is probable that it is not to 

 be considered a karyosome, but derived indirectly from them. 



Anaphase living 



Returning to the study of living material, we find that this can 

 be correlated step by step with that seen in fixed material. The 

 movement of denser portions of disks away from each other does 

 not always occur at once, as strands of the translucent substance 

 connect the separating masses and also strands soon form connecting 

 the granular polar masses (figs. 7, 17-20). These dense strands 

 show no evidence of being composed of homogeneous bodies 

 arranged as strings of beads. This accords with the observations 

 on living material made by Lundegardh (7). 



While the translucent disks that we may term a and b are evolv- 

 ing and gradually retreating from one another, a similar (in appear- 

 ance) jelly-like substance c and d appears at either end of the 

 cylinder as it assumes the typical spindle form (fig. 7)- Disks 

 are represented by light areas. These evidently are not directly 

 derived from the disks, as they are of considerable size before the 

 approach of the latter; also, the disks do not appear to diminish 

 as the strands bordering the cylinder increase. The ends c and d, 

 partly under the chromatophore, show the same optical density, 

 and are translucent and opalescent, while all else is grayish. The 



