256 BOTANICAL GAZETTE [OCTOBER 
That of mutant no. 3 was as follows: 
1908.:..:. Mutant Mutant 
1604-7. Second generation 60 ee 
OTA 5s Third generation 46 per cent cana ~~Lamarckiana Lamarckiana 
It is easily seen that they agree almost exactly with the pedigree 
of mutant no. 1, and simply give further proofs of the conclusions 
drawn from this. The size of the two cultures is given in table IT. 
TABLE IT 
OFFSPRING PERCENT- 
YEAR GENERATION PARENT AGE OF MotTants 
Total Flowering — 
A 
IGOS <<. = WAULONT BOC 3p ee le eo oes els wav cso leet eee ees meer 
Sort... Second mutant 17 16 35 2 nanella 
ak ee Thi a 55 45 15 2 nane 
. 2 new 
TOs... Third Lamarckiana 60 25 Oo ie eee 
1008... POULAIE NG. 2i i oc | ara a en Ee 
1013: : Second mutant 30 30 60 1 lata, ; 
ella 
£944.05. Third cana | 57 56 46 3 nanella 
1644 2; Thir Lamarckiana 60 25 Oo Nee 
In each case the self-fertilized seeds of only one cana were 
studied, besides those of the mutants, and also those of only one 
atavist of the Lamarckiana type. The progeny of the latter . 
proved to be uniform and like the parent, about one-half of the 
plants being studied in the flowering condition and the remainder 
in the stage of large rosettes of radical leaves in July. The /ata 
mutant has flowered, as have the majority of the dwarfs. Two 
mutants of a new type arose, which will have to be described in 
another paper. They resembled O. rubrinervis, but lacked the. 
characteristic brittleness of the stems of this form. 
The second generation of mutant no. 3 has been the most 
vigorous one of all my annual cultures of O. cana. It was grown, 
moreover, under exceptionally favorable conditions. For this 
reason it has been chosen for making a series of crosses, which will 
be dealt with at the end of this article. The fact that, in this case, 
