308 BOTANICAL GAZETTE [may 
initial rosettes. These are recessive in part of the first genera- 
tion, but return in their offspring in one-third of the specimens, in 
about the same way as in the corresponding formula of Mendel. I 
have not tried to go deeper into these questions, however, which 
touch the mutability of O. grandiflora only slightly, but have 
limited myself to two further experiments. 
O. grandiflora ochracea X biennis.—The results of this cross have 
been the same as in the pedigree just given, with the exception that 
the splitting in the first generation fails. The cross was made in 
1914, and the culture of 1915 was a uniform set of 70 plants, most 
of which were very weak and died off before flowering. Only 15 
reached this stage. They grew up like the normal mutant ochracea 
and had from the beginning its slender stems, broad and pale 
leaves. No specimens of the type of biennis were seen. After 
self-fertilization a splitting occurred. Some plants were green and 
stout; the majority, however, were pale and weak. All of them 
had the broad leaves of ochracea, but 9 among 80 made vigorous 
initial rosettes, whereas the remainder grew up without this prepa- 
ration. This gives a percentage of 89 ochracea and 11 biennis. The 
description for both types is the same as in the cross between the 
two species. 
O. grandiflora lorea X biennis.—The narrow leaves of mut. lorea 
are recessive to the broad form of the leaves of the species. In 
other respects the mutant does not seem to differ from it, and thus 
I could expect this cross to give almost the same results as the first. 
I began the experiment in 1914, and in 1915 had the first generation 
with 60 plants, most of which flowered. I counted 9 ochracea with- 
out rosettes, or 15 per cent; the others were of the biennial type. 
Both types agreed in all respects with those of the cross between 
the pure species. 
The second generation from the biennis plants was uniform, 
repeating the type of the parent. I had 7o plants, half of which 
flowered. They were very stout, and already so in the phase of 
rosettes. When flowering, the bed looked almost like pure O. 
biennis L. No ochracea and no lorea were seen among them. 
The seeds of self-fertilized specimens of ochracea of the first gen- 
eration produced in 1916, among 67 plants, a very striking splitting 
