- 
1918] BRIEFER ARTICLES 563 
use xylol to clear and then remove the xylol with 95 per cent and abso- 
lute alcohol; (6) stain in acid fuchsin ro-20 seconds; (7) wash in 
- absolute alcohol; (8) clear in clove oil and xylol; (9) seal in balsam. 
The nucleus is stained a bright red by the safranin, while the cyto- 
plasmic portions of the antherozoid are stained a bluish pink. The 
blepharoplast is more densely stained than the cytoplasmic envelope. 
Sw 
a oe a 4 \ 
Fic. 1.—Antherozoid of Onoclea struthio pteris; 3700 and reduced one-half in 
reproduction. : 
The cilia of the antherozoid are attached for some length along the 
blepharoplast, as shown in fig. 1. No cilia are attached to the extreme 
anterior portion of the blepharoplast. The envelope at the anterior end 
extends a short distance beyond the nucleus, which is small and rodlike 
at this extremity. The nature of the denser portions in the envelope is 
not understood. These were of constant occurrence when the method 
described was used. They could also be readily observed in the living 
antherozoid. Some very good results were obtained when iron haema- 
toxylin was substituted for the safranin—W. N. Stem, University of 
Wisconsin, Madison, Wis. 
