16 ' BOTANICAL GAZETTE [january 



The first culture consisted of 23 laeta and 34 velutina, making a 

 total of 58, with 41 per cent laeta. In 191 5 the figures were 46 

 laeta and 39 velutina, or 54 per cent laeta in 85 specimens. Although 

 the size of the cultures was small, they evidently point to a division 

 in nearly equal groups. The two types were clearly different from 

 the beginning and could easily be counted out in June before the 

 production of the stems. In 191 5 I separated them in March, at 

 the time of planting into the boxes, in order to control my esti- 

 mate later on, and in April planted the laeta in one group and the 

 velutina in another half of the bed. In 19 14 I had 25 and the 

 following year 10 flowering plants, half of which belonged in each 

 case to the laeta type and the other half to the velutina type. The 

 laeta have already been described; the velutina were in no respect 

 and at no time different from ordinary O. blandina. 



The second generation from seed of one of the velutina plants 

 embraced 30 flowering and 40 younger specimens, all of which 

 exactly repeated the marks of their parent. From the seeds of one 

 self -fertilized laeta, however, I got the splitting group already 

 described. Its two types were the same as in the previous gen- 

 eration. I recognized the splitting in the seed pan, but counted 

 them only in June after planting out 15 laeta and 15 velutina. All 

 in all I had 80 plants, of which 26 were laeta and 54 velutina, or 

 33 per cent laeta, which is somewhat less than in the first generation. 

 All the 30 specimens of the bed richly flowered and ripened their 

 first fruits before being thrown away. 



O. LamarckianaXO. blandina. — A biennial plant of the species 

 was crossed in 19 13 with a green individual of the mutant. The 

 seeds were sown partly in 19 14 and partly in 191 5. They gave 

 the same two types as in the reciprocal cross. During the whole 

 lifetime there were no visible differences. In the first year I had 

 60 plants with 22 per cent laeta, and in the second year 108 speci- 

 mens with 25 per cent laeta; the remainder were velutina.' Of 

 these, 25 and 10 flowered, in about equal groups for both types, 

 having been recognized and sorted out at the time of planting. 

 The other plants were cultivated till the end of June. 



O. blandinaXO. nanella. — Two green individuals were ferti- 

 lized in 1 9 13 by the pollen of my race of O. Lamarckiana mut. 



