412 BOTANICAL GAZETTE [may 



desiccator and allowed to concentrate according to the directions given 

 for the Venetian turpentine method. 



Should it be desired to use some of the other stains, such as the 

 Magdala red-anilin blue combination recommended for algae, it will be 

 necessary to modify this manipulation to suit the method. Since these 

 stains are used in strong alcoholic solutions, the material to be stained 

 is washed after killing by the method already described, and then 

 dehydrated by the glycerine method before staining. The glycerine 

 is washed out with 95 per cent alcohol and the stains applied. 



Summary 

 . Treat the material the proper length of time in a suitable killing 



solution. 



2. 



Filter the material to remove killing solution, leaving the material 

 on the filter paper in the funnel. 



3. Wash with distilled water from a wash bottle. 



1 



4. Treat with a 0.1 per cent (or less) iron-alum solution. 



5. Wash with distilled water, using wash bottle. 



6. Stain by application of o . 1 per cent (or less) aqueous haematoxylin 



stain. 



Wash 



8. Differentiate the stain with 0.1 per cent iron-alum solution, 

 washing with distilled water very thoroughly after the treatment. 



9. Dehydrate with glycerine and mount by Venetian turpentine 

 method. 



10. Vary the treatment, when alcoholic stains are to be used, by 

 dehydrating before staining. — J. Ben Hill, Pennsylvania State College, 

 State College, Pa. * 



THE BOTANICAL STATION AT CINCHONA 



The Botanical Station at Cinchona, in the Blue Mountains of 

 Jamaica, which from 1903 to 19 13 was leased by the New York Botanical 

 Garden, has now been leased by the Smithsonian Institution, on behalf 

 of 14 American botanists and botanical institutions that have con- 



tributed the rental. 



that 



counterpart 



zorg Garden of Java. They hope that the opening of this laboratory at 

 Cinchona may prove as stimulating to the development of botany in 



' 



