482 



BOTANICAL GAZETTE 



[JUNE 



latter was then dried in a vacuum desiccator, placed in extraction 

 shells, and extracted with acetone in a Soxhlet extractor; the 

 weight of the lipoid material was determined by evaporation. 

 The nitrogen content was measured by the Kjeldahl method, and 

 the protein content by the Van Slyke method, both of the latter 

 methods as described by Mathews (3). An attempt was made 

 to determine the phosphorus content of the various portions, but it 

 was found to be very low, and insufficient material was left for 

 conclusive analyses. The results of the analyses are summarized 



in table VIII. 



TABLE VIII 



Results of analysis of seed coats of Arachis hypogaea; distribution of 



MATERIALS 



Preliminary 

 extract 



Hot water 



extract 



(gm.) 



Acetone 



extract 



(gm.) 



In residue 

 (gm.) 



Total solids 



Lipoids 



Nitrogen t 



Tannins, equivalent to 



cc. KMn0 4 



1970.0 cc 





3 • 8940 

 0. 2646 



0.0507 



1245.0 cc 



0.6170 

 0.6170 



0.6784 



In sample 

 (gm.) 



0.8816 

 O.7615 



/ 



* Weight of sample, vacuum dried, 30. 1166 gm. 



t Amino acid nitrogen not present in determinable amounts. 



* 



The following points with reference to table VIII are of interest. 

 The water extract contains two substances that are believed to be 



and lipoids. 



permeability of the membrane 



Reicharb (5) 



importance in the permeability 



may 



meability of the peanut seed coat to water. Remembering the 



effect of the acetone extractions in increasing the permeability 



of the membranes, it is interesting to note the low content of lipoid 



A small 



matter 



seems 



5 



mo\ 



From 



maj 



conclude that a part of the increase after 



remov 



The lack 



membrane 



