2 BOTANICAL GAZETTE [JULY 
Methods 
For that part of the work being conducted at Missoula, Mon- 
tana, the opportunities are very favorable. Practically all the 
species of Razoumofskya of any economic importance are of easy 
access from the laboratory. Members of the field force of the 
United States Forest Service are aiding in the work by sending in 
fresh mature specimens of R. pusilla on spruce and larch from the 
Lake states, and of the rare unclassified forms occurring on white 
and yellow pines in Oregon, Idaho, Utah, and Nevada. A great 
deal of material of the common forms from all parts of the North- 
west has also been contributed. The writer visits regularly the 
various forests of the Northwest and has made abundant collections 
of the mistletoes of these regions. The writer is under particular 
obligations to Professor W. C. WErR for service in connection with 
cultures at Bellingham, Washington; to L. H. Werr for collecting 
special material; to D. R. BREWsTER of the Forest Service Experi- 
ment Station, at Priest River, Idaho, and to J. Duncan, Super- 
intendent of Parks of the city of Spokane, for permitting cultures to 
be made on various exotic conifers; and to E. E. Huserr of this 
laboratory for assistance in making cultures. 
From 1911 to 1914 inclusive the inoculations were conducted in 
the open. Seeds were sown on trial hosts of species other than 
that on which they developed, either in the same vicinity or in 
widely separate regions. In the latter case trial hosts of the same 
species as that on which the mistletoe grew were also included. 
This served to check the viability of the seed, also to bring out 
differences due to change of environment between the plants 
resulting from inoculation on the same host species and the plants 
furnishing the seed. The same was true for the plants on trial 
hosts other than that on which the parent plant developed. This 
double procedure demanded copious notes on the conditions of 
growth and general morphology of the plants furnishing the seed 
used in inoculations in other regions and the saving of specimens 
of both sexes for comparison afterward. The same was done with 
plants resulting from inoculation. In the latter case, where neces- 
sary, the infected branch or stem was cut out to prevent the spread 
_of the parasite in new regions. A large number of specimens are 
