52 ee BOTANICAL GAZETTE [juLY 
GiucosE.—(1) Fluckiger’s reaction: yellowish-red precipitate 
of cuprous oxide after heating 1-2 minutes; (2) phenylhydrazine 
reaction: yellow osazone crystals formed after about 24 hours. 
SucROSE.—Remove fructose and glucose. Invert with hydro- 
chloric acid. ‘Test for glucose and fructose as preceding. 
NitTRATE.—(1) Diphenylamine sulphuric acid reaction: blue 
color slowly changing to brown-yellow; (2) brucin-sulphuric acid _ 
reaction: red color. 
OxIDASES.—Benzedine reaction: blue or purple precipitate if 
tissue is acid, soon changing to brown; brown precipitate at once 
if tissue is neutral or alkaline. 
Iron.—(r1) Berlin blue reaction: sections in 2 per cent solution 
of potassium ferrocyanide 15 minutes, add a drop of 2 per cent 
hydrochloric acid. A dark blue precipitate indicates the presence 
of ferric ions. Similarly a red color with potassium ferricyanide 
indicates the presence of ferrous ions; (2) sodium thiosulphate: 
red color. 
MANGANESE.—Sections in 0.1 per cent hydrochloric acid, add 
0.5 per cent sodium ammonium phosphate and ammonia vapor: 
ammonium manganese phosphate crystals, brown color in a 2 
per cent solution of potassium permanganate. 
Matic Acip.—(1) Silver nitrate: sphaero crystals of silver 
nitrate, soluble in ammonia; (2) lead oxide: lead malate crystals; 
(3) sublimation: concentrated sulphuric acid, heat to 130°C.; 
slight charring. 
OXxALIc AciD.—(1) Uranium acetate: large yellow crystals 
of uranium oxalate; (2) strontium nitrate: strontium oxalate 
crystals; (3) ferrous phosphate: yellow precipitate of ferrous 
oxalate. 
AMINO Acips.—Crystallization: treat sections with absolute 
alcohol, crystals of amino acids; (1) compare with known crystal 
form; (2) specific reactions. 
TyROSINE.—Millon reaction: red color. 
ARGININE, HISTIDINE.—Picrolonic acid: yellow crystalline 
precipitate. 
Lreuctne.—Sublimation at 170°C. 
» ASPARAGINE, GLUTAMINE.—Quinone: red color. 
