1918] NOTHNAGEL—FERTILIZATION 159 
6. Two distinct spirems, followed by two groups of chromo- 
somes, arise from the maternal and paternal chromatin in the 
fertilized egg. 
7. There is no complete intermingling of chromatin at fertiliza- 
tion. 
To Professor D. M. Mortier of Indiana University, under whom 
the greater part of this work was done, I wish to express my appreci- 
ation for the helpful criticisms and help given; to Professor C. B. 
ATWELL and Professor W. WoopBuRN of Northwestern University 
for the courtesies and encouragement given while working in their 
laboratory; and to Professor C. J. CHAMBERLAIN of the University 
of Chicago for the suggestion of this problem. 
INDIANA UNIVERSITY 
BLoomincton, INp. 
LITERATURE CITED 
Lal 
. BLACKMAN, V. H., and Wetsrorp, E. J., Fertilization in Lilium. Ann. 
Botany 27:111-115. 1913. 
. COULTER, JoHN M., The endosperm of angiosperms. Bor. Gaz. 52:380- 
386. Iort. 
- Ernst, A., Chromosomen reduction, Entwickelung des Embryosackes, 
und Befruchtung bei Paris quadrifolia und Trillium grandiflorum. Flora 
91: 1-36. pls. I-6. 1902 
4. FerGuson, M. C., The development of ae ig and the fertilization in 
Pinus Strobus. Rhi Botany 15:435-470. 
, Contributions to the knowledge of te ite history of Pinus, etc. 
Proc. Wash. Acad. Sci. 6:1-202. pls. 1-24. 1904. 
GuicNnarp, L., Nouvelles études sur la Shiantation: Ann. Sci. Nat. Bot. 
VII. 14:163-296. 1891 
7c eon, Sur bes astharoktiides et la double copulation sexuelle chez les 
végétaux Angiospermes. Compt. Rend. 128:864-871. 1899. 
8. , Nouvelles recherches sur la double cnoEe chez les végétaux 
‘Aigicmpetnnés: Compt. Rend. 131:153~-160. 
9. ———, L’appareil sexuel et la ces fécondation dans les Tulipa. Ann. 
Sci. Nat. Bot. VIII. 11: 365-387. 
, La double tdoniilbtion he les Cruciféres. Jour. Botanique 
16: ees aes 1902. 
- Heatitey, Marcaret, A study of the life may of Trillium cernuum L. 
Bor. Gaz. 61:425-430. 1916. 
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