262 - BOTANICAL GAZETTE [SEPTEMBER 
the resting stage, but remain in the condition characteristic of a 
telophase. 
Recently Sax (22) has investigated Fritillaria pudica. In most 
cases it is not until the male nucleus and the egg nucleus have 
completely fused that he finds any appearance of the formation of 
the spireme. In rare cases, however, the spireme stage is found 
while the 2 nuclei are still distinct in outline. He believes that the 
rare appearance of such cases is probably of little significance, since 
it is probable that these nuclei subsequently fuse completely because 
no later stages were found in which fusion was incomplete. From 
the many stages and abundant cases of triple fusion he observed 
he thinks there is no doubt that the 2 polar nuclei and the male 
nucleus fuse completely and that the subsequent division is normal. 
Methods 
Stages in fertilization were obtained from ovaries of Liliwm 
philadelphicum collected in the field near Osborn, Calumet, and 
Pine, Indiana, in June and early July, 1916, at the time when the 
petals ‘‘snapped,” and after the petals had fallen. To correlate 
the time of pollination with stages in fertilization, flowers were 
brought into the laboratory, pollinated, and kept under bell jars 
for several days, until fertilization had taken place. In general, 
it may be said that the petals drop on the third day after pollination, 
and the style separates from the ovary on the fourth or fifth day. 
The male nucleus was in contact with the egg nucleus from 60 to 72 
hours after pollination. 
The material for Lilium longiflorum was obtained from plants 
grown in the greenhouse. It produced seeds readily, although it 1s 
generally reported not to set seed. The male nucleus was in contact 
with the egg nucleus about 120 hours after pollination. Of the 
upward of 500 cases of fertilization observed in these 2 species, the 
majority showed the male and egg nuclei in contact, with their 
chromatin in early prophases of the division. 
Chrom-acetic-osmic and Flemming’s medium solutions were 
used as fixatives, and the ovaries trimmed so as to permit more 
rapid penetration of the embryo sacs. Sections were cut 10# 
thick and stained with Flemming’s triple stain or Haidenhain’s 
iron-alum-haematoxylin. 
