252 BOTANICAL GAZETTE [SEPTEMBER 
Mycelia growing in a congo red 1 to 200 parts maltose solution were 
examined to see whether the dye had accumulated in the vacuoles or 
protoplast of the cell. The mycelium had not behaved with methylene 
blue as with the two colloids, but this gave no indication as to the deposi- 
tion of the pigments. : 
The fungus grew in the solution, but not vigorously. The mycelium 
was so deeply stained as to be reddish black. Under the microscope 
the conidia and older cells of the hyphae were dark red, while the youngest 
cells were a pale pink. Treatment with 50 per cent nitric acid showed 
by its blue colored reaction that most of the pigment was in the walls of 
all the cells, only less in the younger cells. The hyphae were plasmolyzed 
with a NaCl solution and also by drying; the contracted protoplasm in 
the center of the cells was red, the cell wall looking white in contrast. 
This first was noticed in the younger cells, the quantity of pigment in 
the older cell walls having obscured the color of the protoplast, until the 
‘last stages of plasmolysis had been reached. Sulphuric acid, nitric, or 
hydrochloric produced besides a blue color, what was thought might be 
_ a blue precipitate. These very small spots, seen with the oil immersion 
lens, were on the cell walls and inside on the plasmolyzed protoplast. 
Glycerin caused the color to stream from the mycelium. Sodium 
hydroxide, while it brightened the red, also caused the color to diffuse 
_ into the surrounding solution. Throughout all these reactions glistening 
white granules in the protoplasts could be seen. 
It would seem from these reactions that a great deal of the congo 
red accumulated in the cell walls, some passed inside the cell walls, where _ 
it appeared as though the protoplast had stored the dye in the form of 
minute granules. In the nutrient solutions containing congo red, the 
difference in ee eeity of hing to store stain was so marked that con- 
- ; t once. For instance, Penicillium sp., yeast, 
and a rod-shaped bacterium f 1 growing in them remained unstained 
until dead. 
It is suggested that Endothia parasitica (Mur.) A. and A. may bea 
- good subject for Se ee 
ee us RUMBOLD, Pinter, al L 
