1917]. CURRENT LITERATURE 257 
The amyl alcohol test for anthocyans has played an mapesiant Part in the 
course of WILLSTATTER’s work. The anthocyan (glucoside) remains quanti- _ 
tatively in dilute (2 N) sulphuric acid when shaken with amy] alcohol, whereas 
the anthocyanidin (non-glucoside) passes quantitatively into the alcoholic 
"layer, yielding a red solution. When shaken with a sdlution of a enone 
the red color becomes violet or violet red, th 
in the amyl alcohol. On shaking with sodiuia ‘carbonate, the alcohol solution 
turns blue or bluish-green, and at the same time the pigment descends quanti- 
tatively into the aqueous layer. 
This is true only for diglucosides. Monoglucosides yield a certain amount © 
fas es Sc » the caaseedh ee Rhamnose glucosides (containing one 
) behave like the monoglucosides. From 
thie ss is seeks the test may be used to distinguish nome- and rhamnose- 
glucosides from diglucosid 
The test is also of service in testing the individuality of anthocyan. For 
this the acid used must be of sufficient concentration to prevent the conversion 
of the colored chloride into the colorless carbinol (W base) and yet must be 
dilute enough to dissolve the chloride readily (0.5 per cent hydrochloric acid 
Two successive extractions with amyl alcohol are made, and the fraction of the 
sp aridcnin we mia agen Mace accia sere Scena wpe h ad 
in both cases 
: gi comprar: i solo ed 
three classes were found. Iti true thatthe possiblity of other clases is, again, 
not excluded. I 
preliminary work 27 
isolation of several new a anthocyanidins may be expected. The one flower 
studied ed. however ae 
ae ia ge pom nd and ni te S 
: formula of chloride, the source from which it —S : fe 
They ai 
ad aan 
