eventually develops i 
1917] HOTSON—BULBIFEROUS FUNGI 279 
definitely traced for some distance inside the filaments of Ascobolus 
magnificus, we are led to the conclusion that the fungus under 
consideration is parasitic on the latter rather than that the bulbil 
is the imperfect condition of it. On all the cultures made of 
P. magnifica the mycelium grew very sparingly, being procumbent, 
and at times growing down into the medium, but never becoming 
flocculent or aerial. On potato, bran, prune, and cornmeal agar 
only a small amount of mycelium was produced even after several 
months. So meager was the development that it might easily 
have been overlooked unless examined carefully with a hand lens. 
Of the different media tried, a decoction of horse dung with agar 
or the horse dung itself, sterilized in an Arnold’s steam sterilizer, 
proved the most satisfactory. 
A microscopic examination frequently showed the mycelium 
to be a network of anastomosing hyphae (fig. 69), while at other 
times (figs. 65-68) enlarged food storage cells were found, the 
largest being 15 » in diameter. 
DEVELOPMENT OF BULBIL.—The primordium of the bulbil is 
quite easily recognized as a short lateral branch, somewhat coiled _ 
or curved and well filled with copmurond ee vi cae develop- . 
ment the bulbil seldom, if ever, p , such 
as does P. parasitica, which it acet closely resembles. From the 
_ end of this coiled branch a cell is cut off, enlarges, a becomes 
well filled with granular food material (figs. 54, 55). This cl 
a PO ee | bil. © ; 
nt tha 
= Aas 
eda tis iced beac oes oo all epresel . 
Wes: St: <5. wile ut other Gel « secondary Wrctic & renee 
from it (figs. 50, 52). The usual mode of procedure, however, is ee 
that Shown i in see te 49» 54, 55- At may be seen that the end © oo 
