280 BOTANICAL GAZETTE [ocTOBER 
laterally. In the course of development these outer cells lose their 
protoplasmic contents, although the walls retain more or less of 
the brownish color. 
Although the foregoing description of the mode of develovae! 
of the bulbil is the usual one, not infrequently a second large cell 
is formed by the primordial branch (figs. 62,63). In such instances 
the further development is practically the same as where there is 
a single central cell. The lateral branches which eventually become 
the cortex are produced from both the large cells, which subsequently 
become completely surrounded, precisely as in the case already 
described. | 
Germination of bulbil 
The bulbils of most of the species of Papulospora germinate 
with little difficulty. All of those described in this article, with 
the exception of P. magnifica, have been found to produce rs 
tubes quite readily. In the study of that species various 
were employed in the hope that a favorable condition might be 
ound for the germination of the bulbils. Among these were 
bran, potato, and prune agar, various synthetic media, as well 
as decoctions of horse dung used both as a liquid and associated 
with agar, but all these failed to produce the desired result. 
Finally a method that the writer had found successful in in- 
ducing the ascospores of certain species of Ascobolus and Cubonia — 
(6) to germinate was tried with some success. Mature bulbils 
were put on a flamed glass slide and carefully crushed with the 
‘flat surface of a scalpel. They were then transferred to hanging 
drops of nutrient media, a sterile decoction of horse dung proving : 
the best. Many of the bulbils thus crushed were totally destroyed, 
_ but in a few instances, where the pressure was just sufficient to 
_ break the cortical layer of cells without injuring the large central i 
one, germination was ‘produced and a branching filament soon 
developed (fig. 39). ce 
7 "The mature ball of P- magnifica, with one or two large ceminal 
? os surrounded by empty cortical ones, superficially resembles - 
co sate ste of 2. enone ay, Hotson. The latter, el : 
