* 
1917] FAUST—RESIN SECRETION 465 
In the tests en bloc equal portions of Balsamorrhiza roots (alco- 
holic preservation of August material) and sprouting stem buds 
(fresh March material) were each placed in 5 cc. of chloroform and 
left for two days. The plant tissues were then removed and 5 cc. 
of sulphuric acid added, according to the Salkowsky-Hesse method, 
and the mixture thoroughly shaken. The results are given in 
table V. 
TABLE V 
| August root | March bud 
Sulphuric acid solution.................. Pale tan Colorless — 
Cionotorin sobitiog 9c. cet cs < Colorless Colorless 
Residue from evaporation............... Colorless Lavender to violet 
MINGPRCPNES 5d oa a None Marked entices eiction 
The Mach test (modified) was used on sections of rapidly grow- 
ing stem buds, just previously placed in 85 per cent alcohol. Sec- 
tions of this material were cut in 95 per cent alcohol; 1 cc. of this 
alcohol, 1 cc. of ferric chloride, and 1 cc. of hydrochloric acid were 
mixed and the sections transferred to this mixture on a depression 
slide. The slide was then gently heated until the mixture was 
reduced to about rcc. Even from gross inspection a typical Mach 
test was produced in the vascular tissues. Examination under low 
power of the microscope showed reactions in the following places: 
heavy stain in the cambium and rays (identical with regions testing 
heavily for resin in fall tissues); specially marked test against walls 
of endodermis facing cortex; all through cortex and pith to more or 
less degree. In the heavily testing regions masses of monoclinic 
crystals were found, deeply impregnated with the stain from sur- 
rounding crystals that had dissolved (fig. 37). This same test was 
applied to roots of the August collection, preserved in 60 per cent 
alcohol. The results of the test were negative. This very 
specimen block had been used previously for resin tests and 
had yielded a decided resin test in the vascular and conductive 
areas. 
These two tests, the Salkowsky-Hesse and Mach, modified to 
meet the needs of the material under investigation, applied to 
Balsamorrhiza material, showed a negative test for fall roots and 
