466 BOTANICAL GAZETTE [DECEMBER 
a uniquely strong ‘positive test for the spring bud region. In 
fact, a comparison of the former test with the present one would 
indicate a much higher percentage of the resene in the spring bud 
than was necessary for a test reaction. Moreover, the Mach test 
both checked up the results obtained in the Salkowsky-Hesse re- 
action and gave the precise location of the resene in the grow- 
ing bud. 
A final test to check up the previous determinations consisted 
in placing some of the material of the fall collection and the spring 
collection in absolute alcohol-ether, half and half, for a period of 
two days, then allowing the filtered solution to evaporate. No 
crystals were found from a careful examination of the fall roots, 
yet an abundance of crystals of the monoclinic type were secured 
from the spring stem material. 
The evidence secured from these reactions for tissues of B. sagit- 
tata shows (1) that resene is found in the growing plant tissues, in 
the meristem and conductive areas; (2) that resene is found in the 
same region in spring tissues where resinic acid is found in the fall 
tissues; and (3) that resinic acid areas in fall tissues test negatively 
for resene. 
In the middle of May roots dug about May 1 were tested for 
percentage of ether extract. Such data are recorded in the ecologi- 
cal section of this paper. This material shows both resene and 
resinic acid present in tissues at this particular time of the year, 
when the leaves had been well developed and metabolic processes 
were near the zenith point. 
When tests were made on various parts of the plant to discover 
whether a Mach test could be secured, the test was negative. 
These tests were made on stem and root tissue and on cotyledons 
and embryo within the seed coat. Later certain crystals were 
noted in the connective and storage tissues of the plant, spheroidal 
in shape, with rays arising from an eccentric umbo. The crystals 
were observed in material which had been preserved in alcohol 
en bloc. These crystals did not occur in fresh aqueous mounts nor 
in fresh material sectioned and mounted in alcohol. The type of 
the crystal was such and its reaction to reagents such as to establish 
