470 BOTANICAL GAZETTE [DECEMBER 
was produced in the respiratory tract, centering in the bronchi, 
giving rise to coughing which seemed almost to split the head. This 
condition continued for almost 36 hours, at the end of which time 
the fever began to abate and the acute pains to leave. The irrita- 
tion of the lungs and bronchi continued for more than a week before 
it was relieved. 
A similar test was made, except that the windows were wide 
open and a strong breeze blew the vapors away from the writer. 
No ill effects were observed. Again, when the apnerates was 
thoroughly hooded, no harmful effects were felt. 
In steam distillation, where water and resene distil and con- 
dense simultaneously, the resene collects on top of the distilled 
water. It was found, however, that a fine grade of filter paper does 
not free the filtrate of the resene product, evidently due to a colloidal 
suspension of the resene in the water. When exposed to the air 
and allowed to evaporate for some time, some of the suspensoid is 
precipitated in crystal form. The toxic effect of this colloidal sus- 
pensoid of resene is brought out by the effect on the protoplasmic 
flow of Chara. 
Resene was distilled from fall roots of B. sagitiata.and allowed 
to condense at 10° C. along with water. This water was filtered 
and the filtrate allowed to act on Chara sp. It tested tannin free. 
The normal time flow for a unit distance was found to be 10, 10, 
10, 10, 10, 10, etc. ‘The Chara was then transferred to this colloidal 
suspension, in parts one of the suspension to nine of water free from 
the suspensoid. Observations on the effect of the resene on the 
protoplasmic flow were made for 90 minutes. The observations 
are recorded in table VI. 
A study of the data shows a marked lowering of the vitality 
immediately upon the introduction of the resene, followed by 
increased activity of the protoplasm. The lowering of the proto- 
plasmic flow occurred almost rhythmically, followed by an alternate 
thythmic increase in vitality. This continued until the final decrease 
in flow, with death ensuing. The amount introduced was much 
less than that required for an appreciable exosmosis (text fig. 2). 
The writer recognizes that a correct quantitative measurement 
of the toxicity of resene and resinic esters is desirable, and has under 
