1 64 BOTAXICAL GAZETTE [august 



nucellar tissue which brings the embryo sac in contact with the 

 inner integument; (7) antipodals ephemeral 



Contrasting features are as follows: (1) the number of ovules 

 is always 2 in Trifolium pratense, but more than 2 and various in 

 the other species; (2) the third megaspore sometimes functions 

 in T. re pens; (3) in Trifolium the embryo sac rapidly destroys 

 the antipodal end of the nucellus and thus forms a long tubular sac; 

 (4) in Trifolium the embryo sac remains very vacuolate, while in 

 Vicia and Medicago the sac fills with cytoplasm; (5) polars meet 

 on median line or on inner side of sac in Trifolium, but rest near 

 the egg apparatus; in Medicago the polars meet near the center of 

 the sac and rest near the egg apparatus; while in Vicia they meet 

 on the inner side of the sac and remain some distance from the egg 

 apparatus; (6) in Trifolium and Vicia, the starch appears in the 

 micropylar end of the nucellus and in the inner integument, while 

 the starch fills the sac in Medicago; (7) in Trifolium the proembryo 

 is short and massive and no definite line between suspensor and 

 embryo was made out; more evidence of the separate parts was 

 seen in the more slender proembryos of T. hybridum and T. repens; 

 (8) definite suspensors with multinucleate cells appear in Medicago 

 saliva and Vicia americana; in the former species the suspensor 

 is filamentous, but composed of two superimposed pairs of cells 

 in the latter species; (9) sterilization is most marked in T. pratense. 



Iowa State College 



Ames, Iowa 



LITERATURE CITED 



1. Compton, R. H., An investigation of the seedling structure in the Legumi- 

 nosae. Jour. Linn. Soc. Bot. 41:1-122. pis. 9. 1912. 



2. Coulter, John M., and Chamberlain, Charles J., Morphology of angio- 

 sperms. New York. 1903. 



3. Guignard, L., Recherches cTembryogenie veg 

 . gumineuses. Ann. Sci. Nat. Bot. VI. 12:5-166. 



I. Le- 



figs 



4. Hegelmaeer, F., Zur Embryogenie und Endospermentwickelung von 

 Lupinus. Bot. Zeit. nos. 5-9. pis. 2. 1880. 



5. Hofmeister, Wilhelm, Embryobildung der Phanerogamen. Jahrb. 



figs 



Pa mass ia 



pis. 14-17. 1912. 



Bot. Gaz. 54:307-329 



