56 



BOTANICAL GAZETTE 



[JULY 



off in the number of seeds after-ripened. Several of the seeds that 

 germinated late in the season had rather stunted hypocotyls. The 

 difference in the number of seeds germinated in the light and the 

 dark at these temperatures seems to indicate that light at least at 

 these temperatures had some influence on the after-ripening. 



TABLE III 



No. 



I 



I 



2 



3 

 4 



No. 



SEEDS 



Carpels 



Temp. 



No. 



DAYS 



IN 

 BATH 



Light 



M 



3 



< 



P 



Germination during 



\ 



On Off 



Mar. 



Apr. 



May 



June 



July 



11 



11 



1 

 

 



Aug. 



■ s 



400 

 4OO 

 400 

 400 

 40O 



/ 

 1 



1 

 1 

 1 



9-22 C. 

 9-22 C. 

 9-22 C. 

 9-22 C. 



9-22° C. 



275 

 264 



275 

 275 

 275 



/ 

 / 



/ 



/ 



/ 



40 



8 



9 



5 

 



25 



9 



1 



2 

 



IS 

 12 















II 

 17 



7 

 







1 



3 

 1 











I03 

 60 



7 

 



determine 



the 



J 3 



time 



C. 



temperature for 10 days, when it was removed 



the ice chest at a temperature of about 

 ^nation was kept up until September, or 

 the seeds germinated during this time, alt 



o 



C. This 10-day 



T>in da vs. None 



exposed one-half of that time, or 70 days, to a temperature most 

 suitable for after-ripening. The high temperatures appear to have 



temperatures 



im 



testas in after- 



removed from 50 seeds and the embry 



temperature 



C. 



embryos showed signs of germination 



nd of 

 They 



then removed from 



days 39 of the 50, or 78 per cent of the seeds treated, had germinated. 

 The time required for after-ripening seeds without testas was about 

 one-third of that required for seeds with testas, under conditions 

 otherwise the same. 



In the after-ripening of these embryos the correlation between 

 cotyledons and hypocotyl was made very evident. The embryos 

 to after-ripen must be kept, at a temperature sufficiently low to 

 inhibit growth in the cotyledons. When the embryos were exposed 





