IQI2] 



SKINNER— CREATININE AND CREATINE 



153 



papers cited. Young wheat seedlings were grown in this series of 

 solutions from March 3 to March 15. To one set of the 66 cultures 

 only the nutrient salts were added, to the second set 50 ppm. of 

 creatinine were added to each culture. Every three days the 

 solutions were changed and analyzed. 



When the two sets of cultures had grown for several days, it 

 was noticeable that the creatinine plants were better developed, 

 having broader leaves and longer and well developed roots. This 

 was more noticeable in some of the fertilizer mixtures than in others. 



The total growth made in the 66 cultures of nutrient salts with- 

 out creatinine, designated as normal cultures, was 166.7 grams as 

 against 181 . 2 grams in the case of the 66 cultures with 50 ppm. of 

 creatinine. Putting the normal at 100, the latter becomes 109, or 

 an increase of 9 per cent as an average of the 66 cultures. As 

 already mentioned, the effect was much more pronounced in cer- 

 tain fertilizer combinations, especially those containing no nitrates, 

 or those low in nitrates. The effects of creatinine in these cultures 

 will now be considered in detail. 



Effect of creatinine on growth 



in 



containing no nitrate 



Table I gives the growth of two sets of cultures composed of 



mixtures 



TABLE I 



Showing the effect of creatinine on growth in cultures containing no 



nitr; 



No. 



1 

 2 



3 



4 



5 

 6 



7 

 8 



9 

 10 



11 



PPM. OF FERTILIZER INGREDIENT IN CULTURE 



SOLUTION 



P.O, 



O 



8 



16 



24 

 32 

 40 



48 



56 

 64 



72 



80 



Nft 



o 

 o 

 o 

 o 

 o 

 o 

 o 

 o 

 o 

 o 

 o 



KaO 



80 



72 

 64 



56 



48 

 40 



32 



24 

 16 



8 



Green weight of culture 



IN GRAMS 



Without 

 creatinine 



1.400 

 I.470 



I -05O 



1-527 

 I.490 



1-558 



1-795 

 1.540 



1.444 

 1.400 



1. 100' 



With 



creatinine 





I.576 



2.200 



2.IOO 

 2.000 

 2.200 

 2.408 

 2.328 

 2.400 



2. 2 20 

 2. IOO 

 I. 150 



