1912] 



SKINNER— CREATININE AND CREATINE 



161 



cultures containing varying amounts of phosphate and potash, but 

 no nitrates, the amount of total fertilizer ingredient in each culture 

 being 8oppm. By an examination of the table it is apparent that 

 the growth of each of the creatine cultures given in the last column is 

 considerably larger than the growth of the cultures without creatine 

 given in the fifth column. The total green weight of the cultures 

 without creatine was 16.2 grams against 23.3 grams for the cul- 

 tures with creatine, an increase of 44 per cent. 



TABLE V 



Showing the effect of creatine on growth in cultures containing no nitrate 



No. 



PPM. OF FERTILIZER INGREDIENT IN CULTURE ! 



SOLUTION 



Green weight of cultures 



IN GRAMS 



P 3 5 



NH, 



K,0 



Without 

 creatine 



With 

 creatine 



I 







8 



16 



24 



32 

 40 



48 



56 



64 

 72 

 80 



ooooooooooo 



80 

 72 



64 



56 



48 



40 



32 



24 



16 



8 







I 1 on 



I.709 

 I.948 

 2.130 

 2.370 

 2.470 

 2.400 



2 2 TO 



2 



X 1 



I 

 I 

 I 

 I 

 I 

 I. 



I. 

 I. 

 I. 







.420 



• 558 



■ 579 

 .528 



.500 

 ,670 

 ,628 

 .600 

 .428 



978 



3 



4 



5 



6 



7 



8 



2.420 

 2.450 



2 070 



Q 



IO 



II 



I.090 





^^ > 



In table VI are given the green weights of plants grown in 

 cultures with and without creatine, containing 8ppm. of NH 3 

 as nitrate and varying amounts of P 2 O s and K 2 0, the total 

 constituents being 8oppm. of P 2 O s +NH 3 +K 2 0. 



These figures 



show that the creatine cultures given in the last column are 

 somewhat larger than the cultures without treatine given in 

 the fifth column, but the difference is not nearly so large as 



■ 



in the cultures containing no nitrate given in table V. The total 

 growth of the cultures without creatine was 26.4 grams against 

 29.4 grams for the cultures with creatine, an increase of only 

 11 per cent in favor of the creatine cultures. There was a 

 difference of 44 per cent in favor of the creatine cultures in the 

 case of the solution which contained no nitrate. 



