162 



BOTANICAL GAZETTE 



[august 



The growth in the cultures which contained varying amounts 

 of phosphate and potash and i6ppm. of nitrate was only 3 per cent 

 greater with than without creatine. In solutions containing 

 24ppm. of nitrate the increased growth with creatine was 6 per 

 cent, and in solutions containing 32 ppm. nitrate the increased 

 growth 4 per cent. In solutions containing higher amounts of 

 nitrate the creatine had no additional effect. Thus it appears that 

 the effect of creatine in replacing the effect of nitrate in producing 

 growth is very similar to that of creatinine. 



TABLE VI 



Showing the effect of creatine on growth in cultures containing 8 ppm. of 



NH 3 as nitrate 



No. 



1 

 2 



3 



4 



5 

 6 



7 

 8 



9 

 10 



PPM. OF FERTILIZER INGREDIENT IN CULTURE 



SOLUTION 



PaO 



O 



8 



16 

 24 



32 



40 



48 

 56 



64 



72 



NH 3 



8 

 8 

 8 

 8 

 8 

 8 

 8 

 8 

 8 

 8 



KaO 



72 



64 

 56 



48 

 40 



32 



24 

 16 



8 



Green weight of cultures 



IN GRAMS 



Without 

 creatine 



2.299 

 2.940 

 2.700 

 2.920 



3°50 



3-!5o 

 3.220 



2.500 



2.222 



1.400 



With 

 creatine 



2. 459 

 3.200 



3-3SO 

 3.400 



3.070 

 3-309 



3 -350 



2.854 

 2.800 



1 .600 



5 



emoval 



milarity 



creatine and creatinine in this respect. 

 in the creatinine cultures the removal 



remem 



but a great deal less nitrate 

 creatinine than in the normal ( 



cultures than the normal 

 lisaDDeared from solutio 



experiments the removal of total P 2 5 , NH 



K 



plants in the normal cultures was 1978.3 



mi 



milligrams 



The normal 



cultures removed 471.0 milligrams 

 tures A7A.A milligrams. In the casi 



normal 



