1912] 



CLAPP—ANEURA PINGUIS 



187 



same time on cotton with distilled water and 1 per cent cane sugar 

 lived. On moist cotton in the sunlight the spores died in the one- 

 celled or two-celled stage. Other cultures, therefore, were kept in 



room with 



that 



roughly with that outside, and the light was kept diffuse by the 

 window shade. Sowings were made on sterilized clay, sand, 



m, humus, and sand, and 



Cultures 



on rotten wood were spoiled by Pencillium. Although the pots 

 containing the soils were scrubbed, dried, sterilized over night in a 



with 



many became 



This could 



i come about when spores were taken out for examination 

 accompanying table records some of the data. 



Sow 



ing 



Final 

 examination 



Time 



Medium 



Condition of spores 



March 



23... 



June 23 



3 mos. 



H 2 on cotton 



2-celled to all stages 

 (fungi ) 



April 



15 — 



June 17 



2 mos.-f 



clay 



1-3-celled; majority 2- 













celled 



April 



15... 



June 17 



2 mos.+ 



H 2 on filter over 

 cotton 



majority 2-celled 



April 



6... 



June 19 



2 mos.-f- 



H 2 on filter over 

 cotton 



2-celled 



May 



5... 



June 19 



1 mo. + 



H 2 on filter over 

 cotton 



2-celled 



May 



5... 



June 17 



1 mo. 



soil 



2-7-celled (fungus) 



May 



21.. . 



June 21 



1 mo. 



sand 



2-celled 



May 



21.. . 



June 21 



1 mo. 



1% cane sugar filter 

 on cotton 



2-celled 



May 



29.. . 



June 21 



1 mo. — 



soils 



1-4-celled (Chaetomium) 



May 



29... 



June 21 



1 mo. 



H a O filter on cotton 



2-celled 



May 



29.. . 



June 21 



1 mo. 



filter over soil 



2-4-celled (fungus) 



This rough table shows that the rate of development is variable 

 and slow. The spores of March 23- June 23 were shed in a heap 

 on the moist cotton in the moist chamber containing the plants 

 from the field. Here were found two-celled stages and thalli 

 with branches. Uninfected plants have reached at most 4 and 5 

 cells, while those with 



fungi have mature 



thalli. This differ- 

 ence is apparently due to some change caused by the fungus. 



Leitgeb (19) describes the germination of Amur a pinguis and 

 A. palmata, but figures only the early stages of A. palmata. He 



