68 BOTANICAL GAZETTE [jantjary 



Summary 



Several intermediate forms were found between three species 

 of Verbena which grow on Stony Island, V. angustifolia Michx., 

 V. striata Vent., and V. hastata L., which can be arranged taxonomi- 

 cally between the three species in question. Embryological and 

 cytological studies were made on the three species and on one of 

 the forms intermediate between V. hastata and V. stricta in order 

 to determine the genetic nature of the intermediate. 



From the cytological point of view, nucellar cap, nutritive 

 jacket, and chambered embryo sac are pointed out as the char- 

 acteristic features of these forms. The reduced number of chromo- 

 somes is 4 in V. angustifolia and 6 in the other three. 



It was not possible to decide from the cytological studies whether 

 the intermediate form is a hybrid or not, since both of the original 

 species from which it might be supposed to have sprung were 

 found to have the same number of chromosomes. The chromo- 



i 



some behavior of the intermediate was like that of the two species 

 and entirely normal. Some of its developmental characters are 

 intermediate and some are similar to either V. stricta or V. hastata. 



Normal College 



Hiroshima, Japan 



figs. 59. iJ 



Campbell 



LITERATURE CITED 



the life history of Lemna minor. Bot. Gaz. 27 : 3 7-66 



Proc. Calif. Acad. Sci. 1:1-62. pis. 1-5. 1897. 



3. Cook, M. T., The embryology of Sagittaria lancifolia L. Ohio Nat. 

 7:97-101. pi. 8. 1907. 



4. Coulter, J. M., and Chamberlain, C. J., Morphology of angiosperms. 



1915. 



5 



Ann. Sci. Nat. Bot. 2:37-128. pis. i-j. figs 



GuiGNARD, M. L.. Recherches sur le sac em 



grasses 



Angiospermes. 



136- 



7- — ~ — , La double fecondation chez les Solanees. J 



figs. 45- i9°2. 

 8. Hall, J. G., An embryological study of Limnocha 



Gaz. 33:214-219. pi. 9. 1902. 



Bot. 



