1920] 



SC HERTZ— SUDAN GRASS SEED 



79 





the total reducing sugars were 2 per cent greater, while the carbo- 

 hydrates were over 6 per cent less. Five times as much crude fiber 

 was found in the unhulled seeds. 



A further analysis of the sugars was then made. Two samples 

 of 25 gm. each of the dry seed were analyzed for sugars alone. Two 



24° C.) 



samples of 25 gm. each were grown at room temperature (16- 

 for 3 days, and two other samples were grown in the refrigerator 

 for 32 days. The seeds in each case were extracted as indicated 

 into the two portions F 2 and F 3 . F 2 was then evaporated down and 

 made up to a volume of 500 cc, of which 100 cc. was used for the 

 determination of total reducing sugars; three 50 cc. samples for 

 the inversion of cane sugar by weak hydrolysis at 67-69 C. for 

 10 minutes; and the remainder was used for free reducing sugars. 

 All of the F 3 was hydrolyzed for 2.5 hours by adding 300 cc. water 

 and 30 cc. hydrochloric acid (sp. gr. 1 .125). From small portions 

 of this the total sugars of F 3 were determined. 



From table IV it is seen that when the seeds germinate the 

 sucrose-like sugars decreased about 1 per cent, while there was a 

 decrease in the total carbohydrates of about 15 per cent. 



TABLE V 



Sudan grass compared with other seeds 



Seeds 



Triticum sativum 



Hordeum sativum 



Secale cereale 



Zea Mays 



Sorghum saccharatum. . 



Oryza sativa gg^ 

 A vena sativa 



Water 



Protein 



Fat 



Holcus halepensis sudanensis te 1 }^ 



Unhulled germinated seed in refrigerator . . . 

 budan grass seed (Kansas)J 



10.93 

 10.01 



11 .19 

 9-10 



944 

 813 





10.25 



6.44 



8.4 



746 



13.69 





1.65 

 1.87 



1.68 



4-5 



318 



1.27 



2.00 



527 



3.60 

 4-48 

 3-6o 



3.8i 



N-free 

 extract 



70.01 

 67.88 



69.36 

 68-69 



68.55 



7S-50 





59-68 



75 • 74* 



71.43* 



59 • 78* 

 63-63 



Crude 

 fiber 



2.12 



423 

 2.16 



1.6-2.7 



2.54 

 0.88 



Ash 



997 



4-95 

 I 03 

 5.08 



5-38 



1.92 

 306 

 2.24 

 1.60 



1. 71 



1-03 



3-57 

 3 02 



524 

 2.16 



5-36 



309 



Sugar 



2-7 

 6-7 



1-5-3-7 

 1-2 



2-5 



2.39t 



3-44t 

 2.69! 



* 100 — (protein +ether extract -f-ash-f moisture -{-crude fiber). 



t Total reducing sugars as dextrose. 



J Thompson, G. E., Sudan grass in Kansas. Kansas Agric. Exper. Sta. Bull. 212. 



1916. 



It is of interest to compare these results with those of some other 

 workers. Kjeldahl, working on barley seed, found about 4 . 7 per 

 cent cane sugar in the green malt and 1 . 1 per cent in the ungermi- 

 nated barley. O'Sullivan found in ungerminated barley 0.8-1 .6 



