1920] 



SC HERTZ— SUDAN GRASS SEED 



81 



the micropylar end of the caryopsis. With acetone and a drop 

 of concentrated HC1 a red color was noted on the pedicel, and 

 especially was the red prominent in the whole pericarp integu- 

 ment. This indicated strongly the 

 presence of methyl pentosan, and per- 

 haps araban and xylan. No callose 

 was observed in any of the tissues. 

 With ruthenium red, the pericarp in- 

 tegument and the cell membranes of 

 the starchy endosperm gave slight 

 tests, while the scutellum, plumule, 

 plumule sheath, radicle, and root 

 shoot gave a strong reaction, indicat- 

 ing the presence of much pectic sub- 

 stance. Small particles in the cells 

 also gave a pectose reaction. The 

 phloroglucin-HCl tests showed only 

 traces of lignin, if any, present in the 



— 



pedicel and in the glume. 



Upon 



f*~ 



m V 





heating the tissues with concentrated 



HNO3 an d concentrated KC10 3 , eerie 

 acid was observed to issue from the 

 tissues of the pericarp integument. 

 Suberin was present here. 



All cells of the embryo, and espe- 

 cially the cells of the embryo at the 

 micropylar end, were rich in oil. 

 The fat-containing cells of the endo- 

 sperm stained heavily with Sudan 



III. Also, the epithelial layer had of grain of Sudan grass: a, glume; 



- b, pericarp; c, aleurone layer; d, 



some fat present. The whole of the endoS p erm ; h scutellum; /, cole- 

 embryo became red when treated with optile; g, plumule; A, embryo node; 



Concentrated H 2 S0 4 , and later took a *\ radicle; i, root cap; *,coleorhiza; 

 nr^ • * , TT , , /, pedicel; m 7 basal seta; «,glandu- 



greenish hue. Hence, phytosterol la H aye r of scutellum; ., lodicule. 

 was thought to be present in the 



embryo, and also in a portion of the seed coat at the micropylar 



end of the caryopsis. 



Fig. 1. — Longitudinal section 



