1920] 



WALSTER— BARLEY 



115 



sugars, and for total sugars after mild hydrolysis. Samples 24 

 and 87 were also analyzed for inorganic phosphorus, using the 

 Powick-Chapin (10) method. F 3 was analyzed for total N, 

 total P, N and P soluble and insoluble in 1 per cent NaOH, phos- 

 phoprotein phosphorus, polysaccharides, and cellulose, etc., by 



TABLE V 



Effect of temperature on distribution of extractives and insoluble matter 



in barley leaves 



44- 

 24. 



41. 

 108. 



87. 

 104. 



Culture no. and treatment 



High N warm 



High N warm 



High P and N warm 



High N cool 



High N cool 



High P and N cool. . 



Soluble in 

 anhydrous ether 



(R) 



Percentage 



8.699 

 7.885 



10.433 

 8.251 



8.663 



7.681 



Soluble in hot 

 alcohol and water 



(Fa) 



Percentage 



33 



33 

 32 



30 

 27 



3° 



017 



743 

 613 



321 



823 



279 



Insoluble in ether, 

 alcohol, and water 



(F f ) 



Percentage 



S8.284 



58.372 



56.954 

 61.428 



635*4 



62.040 



difference. The following list 



gives 



• 



the methods employed. 



The details of the several methods are those recommended by 



Koch (8) and Mathews (9). 



Total nitrogen Arnold-Gunning method. 



Total phosphorus Neuman-Pemberton method. 



Direct reducing sugars. . . Bertrand volumetric method (glucose calculated from 



Munson- Walker tables in Mathew's Physiological 



Chemistry) . 

 Total sugars Bertrand volumetric method applied to the products 



of mild hydrolysis with HC1. 

 Polysaccharides Bertrand volumetric method applied to the products 



Phosphoprotein 



of strong hydrolysis with HC1. 



phosphorus Determination of the P precipitable by Mg mixture 



in an extract made by 48 hours' digestion with 

 1 per cent NaOH at 37-40 C. 



The method for phosphoprotein phosphorus is based upon the 

 discovery by Plimmer and Scott that phosphoproteins can be 

 separated from nucleoproteins through hydrolyzing the former 

 with 1 pe-rent NaOH, the latter being unattacked by the dilute 



