226 



BOTANICAL GAZETTE 



[march 



TABLE V 



I 



Effect of o.ioNMnCl 2 and K 2 S0 4 on oxidation of pyrogallol by precipitated 



OXIDASE FROM BOTH HEALTHY AND DISEASED APPLE BARK; TEMPERATURE 



29. 5-3O. 2° C. 



Time of reading 



June 21 



1 .45 

 2.00 



215 

 2.30 



2-45 

 3.00 



3.15 



3-3o 



3-45 

 4.00 



4- IS 

 430 



4- 45 

 June 22 



8.00 



Check 



O.OO 

 O.07 

 0.08 

 O.08 

 O.08 

 O.08 



O.I5 



O.I5 

 0.18 



o. 20 

 0.20 



0.23 



0.28 



0.53 



Healthy 



MnCla 



O.00 

 0.08 



O.IO 



013 



0.13 



o. 10 



O. II 



0.08 



0.09 



0.08 

 0.08 



0.09 



0.18 

 0.28 



K 3 S0 4 



0.00 

 o. 11 



o 

 o 

 o 

 o 

 o 



21 



23 

 27 



25 

 28 



0.30 



035 



0.34 

 037 



0.38 



0.43 



0.58 



Check 



Diseased 



MnCla 



0.00 



0.00 



0.17 



0.15 



o-37 



0. 29 



0.42 



0. 21 



0.48 



0.25 



0.50 



0.23 



0.56 



0.24 



0.65 



0.26 



0.70 



0. 29 



0.79 



0.31 



0.87 



0.34 



0.88 



o.35 



0.98 



0.40 



1.24 



0.63 



KaS0 4 



O.OO 



O.I5 

 O.3O 



033 



0.43 

 O.48 



0.54 

 O.58 



O.63 



O.69 



O.78 



O.78 



0.93 

 1.28 



2 



I 



Fig. 5.— Effect of MnCl a and K 2 S0 4 on the oxidation of pyrogallol on precipitated 

 oxidase from both healthy and diseased bark: A (diseased), K 2 S0 4 +bark and pyro- 

 gallol; B (diseased), control (bark and pyrogallol); C (diseased), MnCl 2 +bark and 

 pyrogallol; D (healthy), K 2 S0 4 +bark and pyrogallol; E (healthy), control (bark 

 and pyrogallol); F (healthy), MCnl 2 +bark and pyrogallol. 



