1913] HAWKINS—MALT DIASTASE 273 
285/165, 285/150, 285/180, 285/315, 285/285, and 285/300, 
Or 1.73, I.73, 1.90, 1.58, 0.90, 1.00, and 0.95, when the ve- 
locity of the reaction without added salt is taken as unity 
| TABLE II 
EFFICIENCY OF DIASTATIC ACTION AS AFFECTED BY VARIOUS CONCENTRATIONS OF 
SODIUM AND CHLORIDES, SINGLY AND COMBINE 
SINGLE SALTS hapeiaauitas 
Glace proportions, 
NaCl CaCl NaCl 2:CaChL 1) 
: Total salt con- 
Boy Efficiency | Concentration | Eésciency centration in Efficiency 
mixture 
2m ret Mol. Retardation 3m Retardation 
m/2 t.3F ~ m/4 £2393 3m/4 1.21 
m/8 1.82 m/16 2.80 3m/16 3-29 
/32 1.42 /6 3.69 3m/64 3.40 
m/128 0.85 m/25 3.01 3m/256 2.75 
m/512 0.95 m/1024 1.88 3m/1024 1.85 
m/2048 1.03 m/4096 1.07 3m/ 4096 i.17 
TABLE III 
EFFICIENCY OF DIASTATIC ACTION AS AFFECTED BY VARIOUS CONCENTRATIONS OF 
IUM AND MAGNESIUM CHLORIDES, SINGLY AND COMB: 
SINGLE SALTS ComsrnaTIons 
(Molecular proporti 8 oe pen ons, 
NaCl MgCl: NaCl 4:MgCh 1) 
i : Total salt con- 
ie ay Efficiency ye es omer Efficiency centration in Efficiency 
2m 1:2 m/2 Retardation | 5m/z2 Retardation 
m/2 t.91 m/8 25 5m/8 
m/8 1.82 m/32 2.07 5m/32 1.90 
/32 1.42 m/128 1.97 5m/128 2.21 
m/128 0.85 m/512 1:35 5m/512 1.54 
m/512 0.95 m/2048 1.07 5m/2048 0.89 
m/2048 1.02 m/8192 1.00 5m/8192 0.99 
These numbers may be termed the ratios of the enzyme activity 
or of efficiency. Such a computation as the foregoing has been 
made in each case, always with the enzyme activity of the control 
for the particular experiment in question taken as unity, and it 
