1913] HAWKINS—MALT DIASTASE 275 
action of the enzyme. Of course the combination treatments 
possess a total salt concentration equal to the sum of the two indi- 
vidual concentrations of the single salts which have been combined. 
TABLE VI 
EFFICIENCY OF DIASTATIC ACTION AS AFFECTED BY VARIOUS CONCENTRATIONS OF 
AGNESIUM AND CALCIUM CHLORIDES, SINGLY AND COMBINED 
SINGLE SALTS Comanrartons 
. (Molecular proportions, 
MgCl CaChL MgCl 1:CaCh 2) 
Total salt con- 
7 ma year org Efficiency —— Efficiency centration rn Efficiency 
m/2 eae Mol. Retardation | 3m/2 Retardation 
m/8 2.25 m/4 1.33 3m/8 1.06 
/32 2.07 m/16 2.89 3m/32 3.06 
m/128 1.97 /64 3.69 3m/128 3-61 
m/512 1.35 m/256 3.01 3m/512 2.65 
m/2048 1.07 m/1024 1.88 3m/2048 2.09 
m/8192 1.00 m/4096 1.07 3m/8192 1.16 
TABLE VII 
EFFICIENCY OF DIASTATIC ACTION AS AFFECTED BY VARIOUS CONCENTRATIONS OF 
C AND CUPRIC CHLORIDES, SINGLY AND COMBINED 
SINGLE SALTS 
: CoMBINATIONS 
(Molecular proportions, r:1) 
Fe,Cls CuCL 
Co: tratio: : : : Total salt con- " 
in, an — Efficiency a Efficiency german in Efficiency 
m/1024__| Retardation m/1024_ | Retardation [512 Retardation 
m/2048 1.45 m/2048 3.50 m/1024 | Retardation 
m/4096 3.55 m/ 4096 2.62 m/2048 3.17 
/8192 3-91 m/8192 1.26 / 4096 3-59 
m/16,384 1.98 / 16,384 1.00 m/8192 {| ~—si1..92 
m/32,768 1:87 m/32,768 1.00 met 384 1.45 
m/65,536 1.00 m/65,536 1.00 m/32,768 1.00 
The total salt concentrations for the several combinations are given 
in the tables. 
It was impracticable to combine 2m NaCl with 
2m KCl, so this combination was not tested. The molecular 
Proportions at which any two salts were combined are clear from 
