350 BOTANICAL GAZETTE [MAY 
concentrations of the smokes used, along with condition of the 
Les at the close of the experiment: 
. Check (in duplicate) in 10-liter chamber; epicotyls 6-11 cm. tall, ver- 
that ial slim. 
2. Lighted cigarette sealed in 1o0-liter chamber; epicotyls 2.5-3.5 ¢ 
long; swelling o.5-1 cm. long; shorter swelling vertical and longer ones 
declined as much as go°. 
3. Lighted pine splinter in 5o0-liter chamber; epicotyls 3-5 cm. long; 
swelling 1-2 cm.; declination 50°—90°. 
4. Lighted piece of linen paper (not giving lignin test) in 1o-liter chamber; 
epicotyls 3-3. 5 cm. long; swelling knoblike; no declination. 
. Alighted sheet of ashless filter paper (6cm. diameter) in ro-liter 
chacibér. seg 1-2 cm. long; swelling 1-2 cm.; declination 60°-go°. 
6. Two lighted straws (12 cm. long) partly burned in 1o-liter chamber; 
aati 3-3.5 cm. long; swelling knoblike, ‘to rem. long; declination 0°90”. 
. Three whiffs of cigarette smoke in 1o-liter chamber; reaction almost 
Sedid with 6. 
8. o.13-gm. linen paper burned as open sheet in 10-liter chamber; 
epicotyls 3-5 cm. long; swelling 1.5-2.5 cm.; declination 60°-go°. 
g. ©.06-gm. linen paper burned in 10-liter chamber; epicotyls 4-7 cm. long; 
no swelling; diameter everywhere greater than in checks; declination o°-15°. 
10. 0.09-gm. linen paper burned in 1o-liter chamber; reaction about the 
* same as 9. 
I. 1245-gm. linen paper burned in too-liter chamber; epicotyls 2. 5-3-5 
cm. long; swelling o.5-o.75 cm.; slight declination. 
12. 1.45-gm. linen paper burned in 50-liter chamber; reaction about 
as II. 
13. ©. 36-gm. linen paper in 100-liter chamber; epicotyls oe 5 cm. long; 
no swelling, but larger diameter than checks; declination 20°-40°. 
From this experiment it is evident that the smoke from the cellu- 
lose and lignin compounds is rather toxic. In agreement with 
Mottscu, it shows that the toxic effect of tobacco smoke is not due 
to substances peculiar to tobacco, but is as marked for the smoke of 
pure cellulose. The unwashed smoke from 0.13 gm. of cellulose 
in 10 liters gives a response lying between NELJUBOW’s responses 
3 and 4. 
Experiment II.—Effect of washed smoke 
In preparing the smoke, the cigar or cigarette (tobacco or paper) 
was smoked by suction and the smoke washed through two special 
wash bottles, one containing 15 per cent H,SO,, the other 40 per 
cent NaOH. According to LEHMANN (18), the first removes all 
