352 BOTANICAL GAZETTE [May 
4. 100 cc. cigar smoke (output of 0.075 gm. of cigar) in 50-liter chamber; 
epicotyls 3.5-4cm. long, with swollen regions 0.75~1.5 cm. long; swollen 
zones varying from upright to horizontal; the shorter sens upright zones 
indicate that there i is no growth in length. 
$430 cc. sows smoke big at of 0.097 gm. of cigar) in 50-liter chamber; 
many epicotyls showed | llings; no growth in length and no declina- 
tion; many others were dead. 
200 cc. cigar smoke (output of 0.15 gm. of cigar) in 50-liter chamber; 
Sacityis mostly dead; living ones vertical, with a knoblike swelling; no grow 
in length 
7. 330 cc. of cigar smoke (output of o. 25 gm. of cigar) in 100-liter anher 
condition of epicotyls between that of 5 and 6. 
8. 40 cc. of cigarette smoke (output of 0.024 gm. of cigarette) in 1o-liter 
chamber; epicotyls 3-4. 5 cm. long; swollen zone 1-2 cm. long; declination of 
swollen portion 80°—go0°. 
g. 20 cc. of cigarette smoke (output of 0.012 gm. of cigarette) in 1o-liter 
chamber; epicotyls aed cm. long; swollen portion 2-3 cm. long; declination 
of swollen part 70°90". 
occ. cigarette smoke (output of 0.006 gm. of cigarette) in ro-liter 
seers epicotyls 4-9 cm. long; little declination; no swelling; diameter of 
epiotyls greater than that of checks. 
. 20 cc. paper smoke (output of 0.007 gm. of paper) in 1o-liter chamber; 
ecivte 3-4.5 cm. long; swelling 0. 75-1. 5 cm. long; swollen portion vertical 
to horizontal. 
12. Io Cc. paper smoke (output of 0.0035 gm. of paper) in ro-liter chamber; 
epicotyls 4-5 cm. long; swelling 1-2.5 cm. long and declined 70°-go”. 
Several things are evident from these experiments. The smoke 
of paper and tobacco cigarettes is still very toxic after thorough 
washing with 15 per cent H,SO, and 4o per cent NaOH. Later 
data will show that the constituents thus washed out have a com- 
paratively low magnitude of toxicity. It becomes probable then 
that the high toxicity of paper and tobacco smoke is determin 
by the dry distillation carbon-bearing gases. Figuring on the basis 
of 1o-liter containers, 10 cc. of cigar smoke (output of 0.0075 gm.), 
40 cc. of cigarette smoke (output of 0.024 gm.), and rocc. paper 
cigarette smoke (output of 0.0035 gm.) give the third response 
mentioned by NELJuBow (reduced rate of elongation, swelling, and 
horizontal position of the swollen part). 
On the basis of the amount of washed smoke necessary to give 
the response, the cigar and paper cigarette smokes are about equally 
effective, and the tobacco cigarette smoke about one-fourth as 
