354 BOTANICAL GAZETTE [MAY 
This analysis gives a trace of heavy hydrocarbons (bromine- 
absorbed gases) and approximately 15 per cent of carbon monoxide. 
The figures show that each gram of paper produced 263 cc. of CO. 
This yield is very high when compared with the yield from tobacco 
in cigarettes, cigars, and pipe as reported by LEHMANN (18). In 
these it varies from 15 to 101 cc. of CO per gram of tobacco. Our 
figures may be a little high, due to incomplete drying of the stub 
before weighing. 
Tightly wrapped paper cigarette. In this case 3.41 gm. of paper produced 
7357 cc. of washed smoke. 
Analysis - . 
Volume of smoke taken for analysis ............ 07.6 cc cc 
Volume after absorption with 40 per cent NaOH 5.8 90.6 
Volume after absorption with phosphorus ....... 95.8 9 
Volume after absorption with bromine water 95.8 
Volume after absorption with ammoniacal cuprous| 
Pee ene re es ks 81.1 77.9 
ek oe kes eae 14.7 13.6 
In this analysis considerable CO, appears. On the basis of the 
CO,-free smoke the CO constitutes 15+ per cent, approximately 
the same percentage shown in the analysis of the loosely rolled 
cigarette. There is not a measurable amount of heavy hydro- 
carbons, but in an analysis like this, where no corrections are made 
for temperature changes, it is possible to leave undetected o.2 cc. 
In this case each gram of paper produced 327 cc. of CO, an even 
higher yield than given by the loosely rolled cigarette. This too 
is probably a little high, due to insufficient drying before weighing 
the stub back. 
Effect on the seedlings —At the beginning of the experiment the 
epicotyls were 2-3 cm. tall. The cultures were sealed in 10-liter 
cans and subjected to the smoke for 3 days. The. following data 
show the nature and concentration of the smoke used, along with 
the condition of the seedlings at the close of the experiment. 
1. Check; epicotyls 5-13 cm. tall, vertical and slim. 
Washed smoke from loosely rolled paper cigarette 
2, 20 cc. ; cores: 3.5-5 cm. long; swollen portion 1-2 cm. long, with 
declination of 75°-90°. 
